Chicken Induced Pluripotent Stem Cells (BA3)

Chicken Induced Pluripotent Stem Cells (iPSCs) generated using human reprogramming factors for developmental biology and disease research.

Highlights:

  • First nonmammalian iPSCs using human pluripotency genes rather than avian genes
  • Capable of forming all 3 germ layers in vitro
  • Demonstrated differentiation in culture into astrocytes, oligodendrocytes, and neurons
  • Capable of generating live chimeric birds and incorporating into tissues from all 3 germ layers, extraembryonic tissues, and potentially the germline

Induced pluripotent stem cells (iPSCs) are highly proliferative populations capable of differentiating into multiple cell types and tissues representing all 3 germ layers and the germline. Similar to ESCs, mammalian iPSCs exhibit high levels of plasticity in both in vitro and in vivo environments. Avian embryonic models are useful for providing critical new insights into developmental biology including organ function, disease progression, eye disorders, and many others.

From the laboratory of Steven L. Stice, PhD, University of Georgia.

Catalog Number Product Size AVAILABILITY Price Qty
EGA927
Chicken Induced Pluripotent Stem Cells (BA3), 1 Vial
1 Vial In stock
Price: $599.00
Specifications
Name: BA3
Cell Type: Induced Pluripotent Stem Cell
Organism: Chicken
Morphology: Monolayer or Suspension
Biosafety Level: BSL-2
Subculturing: Can be clonally passaged
Growth Conditions: DMEM /F12, 5% KSR, 50 U/ml penicillin, 50 ug/ml streptomycin, 2 mM L-glutamine, 0.1 mM non-essential amino acids, 0.1 M mercaptoethanol in 5% CO2 atmosphere at 37 °C, if culturing in suspension, must be on shaker at 50-100 rpm
Cryopreservation: DMEM /F12, 5% KSR, 50 U/ml penicillin, 50 ug/ml streptomycin, 2 mM L-glutamine, 0.1 mM non-essential amino acids, 0.1 M mercaptoethanol, 10% DMSO
Source: Embryonic Fibroblast
Comments: Media should be changed every 24-48 hours, passaging when 75-80% confluency is reached
Storage: Liquid nitrogen
Shipped: Dry Ice
Provider
From the laboratory of Steven L. Stice, PhD, University of Georgia.
References
  1. Lu Y, West FD, Jordan BJ, Mumaw JL, Jordan ET, Gallegos-Cardenas A, Beckstead RB, Stice SL. Avian-induced pluripotent stem cells derived using human reprogramming factors. Stem Cells Dev. 2012 Feb 10;21(3):394-403.
  2. Shittu I, Zhu Z, Lu Y, Hutcheson JM, Stice SL, West FD, Donadeu M, Dungu B, Fadly AM, Zavala G, Ferguson-Noel N, Afonso CL. Development, characterization and optimization of a new suspension chicken-induced pluripotent cell line for the production of Newcastle disease vaccine. Biologicals. 2016 Jan;44(1):24-32.

If you publish research with this product, please let us know so we can cite your paper.

 
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