Neutrophil Specific, NIR Fluorescent Imaging Agent

Neutrophil-Specific, NIR Fluorescent Imaging Agent

This reagent is a Cyanine7-conjugated, PEG-modified hexapeptide that specifically binds the formylpeptide receptor (FPR) of neutrophils. After tailvein injection of the agent, sites of inflammation, where neutrophils have been recruited and activated, can be imaged in vivo by near-infrared (NIR) fluorescence.

(Left) Representative fluorescence image at 3 hrs post injection of Neutrophil-Specific, NIR Fluorescent Imaging Agent (cFLFLF-PEG76-Cyanine7) (2 nmol): A, normal group; B, blocking group pre-injected with 100 nmol (50 eq.) of a non-fluorescence blocking peptide 1 hr before injection of the Neutrophil-Specific, NIR Fluorescent Imaging Agent.

From the laboratory of Dongfeng Pan, PhD, University of Virginia.

Read related blog post, Development of a Fluorescent Imaging Probe Specific for Neutrophils »

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Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EG0001
Neutrophil-Specific, NIR Fluorescent Imaging Agent, 0.25mg
0.25mg Currently unavailable
Regular Price:$230.00
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Specifications

Product Type: Small Molecule
Name: Neutrophil-Specific, NIR Fluorescent Imaging Agent (cFLFLF-PEG76-Cyanine7)
Molecular Weight: 5 kDa (average calculated mass) 0.25mg = 50nmol
Format: lyophilized powder
Purity: >95%, HPLC
Tested Applications: in vivo imaging
Solubility: Soluble in water and PBS. Create stock solution of 20uM by dissolving 0.25mg into 2.5ml of sterile water or PBS.
Ex/Em Maxima: Ex: 745nm
Storage: Protect from light. Once reconstituted, store aliquoted at -20C for up to 3 months (avoid repeated freeze / thaw cycles). For long term storage, keep lyophilized at -20C for up to 12 months. 
Shipped: Ambient temperature

Data
SKH mice from CRL (6 mice per group) were treated with a strong neutrophil-inducing agent. Neutrophil-Specific, NIR Fluorescent Imaging Agent was then dosed at 0.5 nmol per 20g of body weight. Mice were imaged with an IVIS lumina XR imaging system using the auto settings with medium binning and an F stop of 2. Adaptive FL Background subtraction was performed. (Unpublished data from customer at NIH).
Provider
From the laboratory of Dongfeng Pan, PhD, University of Virginia.
Comments

Suggested Use:
Dissolve Neutrophil-Specific, NIR Fluorescent Imaging Agent in water or neutral buffer for a working solution concentration of 20uM. Inject mice with 50uL Working Solution per 10g body weight via intravenous injection.

  • Inject mice at 2nmol per 20g of body weight.
  • The half life of this compound is between 1.5 - 2hrs
References
  1. L. Xiao, Y. Zhang, M. D. Chordia, S. S. Berr, P. Pramoonjago, L. Pu, D. Pan. A novel near-infrared fluorescence imaging probe for in vivo neutrophil tracking. Mol Imaging. 2012 Sep-Oct;11(5):372-82.
  2. Xiao et al. Synthesis of the Cyanine 7 labeled neutrophil-specific agents for noninvasive near infrared fluorescence imaging. Bioorg. Med. Chem. Lett. 20 (2010) 3515–3517.
  3. Mott TM, Johnston RK, Vijayakumar S, Estes DM, Motamedi M, Sbrana E, Endsley JJ, and Torres A. Monitoring Therapeutic Treatments against Burkholderia Infections Using Imaging Techniques. Pathogens 2 (2013) 383-401. View Article
  4. Pletzer D, Mansour SC, Wuerth K, Rahanjam N, Hancock RE. New Mouse Model for Chronic Infections by Gram-Negative Bacteria Enabling the Study of Anti-Infective Efficacy and Host-Microbe Interactions. MBio. 2017 Feb 28;8(1). pii: e00140-17. doi: 10.1128/mBio.00140-17. PubMed PMID: 28246361; PubMed Central PMCID: PMC5347345. View Article
  5. Manuel ER, Chen J, D'Apuzzo M, Lampa MG, Kaltcheva TI, Thompson CB, Ludwig T, Chung V, Diamond DJ. Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors. Cancer Immunol Res. 2015 Sep;3(9):1096-107. doi: 10.1158/2326-6066.CIR-14-0214. Epub 2015 Jul 1. PubMed PMID: 26134178; PubMed Central PMCID: PMC4561205. View Article
  6. Kandahari AM, Yang X, Dighe AS, Pan D, Cui Q. Recognition of Immune Response for the Early Diagnosis and Treatment of Osteoarthritis. J Immunol Res. 2015;2015:192415. View Article
  7. Xiao L, Ding M, Zhang Y, Chordia M, Pan D, Shimer A, Shen F, Glover D, Jin L, Li X. A Novel Modality for Functional Imaging in Acute Intervertebral Disk Herniation via Tracking Leukocyte Infiltration. Mol Imaging Biol. 2017 Jan 3. doi: 10.1007/s11307-016-1038-6. View Article
  8. Pletzer D, Wolfmeier H, Bains M, Hancock REW. Synthetic Peptides to Target Stringent Response-Controlled Virulence in a Pseudomonas aeruginosa Murine Cutaneous Infection Model. Front Microbiol. 2017 Sep 27;8:1867. View Article
  9. Ivanciuc T, Sbrana E, Casola A, Garofalo RP. Protective Role of Nuclear Factor Erythroid 2-Related Factor 2 Against Respiratory Syncytial Virus and Human Metapneumovirus Infections. Front Immunol. 2018 Apr 23;9:854. View Article
  10. Wu BC, Lee AH, Hancock REW. Mechanisms of the Innate Defense Regulator Peptide-1002 Anti-Inflammatory Activity in a Sterile Inflammation Mouse Model. J Immunol. 2017 Nov 15;199(10):3592-3603. doi: 10.4049/jimmunol.1700985. Epub 2017 Oct 9.View Article

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