|Name:||Neutrophil-Specific, NIR Fluorescent Imaging Agent (cFLFLF-PEG76-Cyanine7)|
|Molecular Weight:||5 kDa (average calculated mass) 0.25mg = 50nmol|
|Tested Applications:||in vivo imaging|
|Solubility:||Soluble in water and PBS. Create stock solution of 20uM by dissolving 0.25mg into 2.5ml of sterile water or PBS.|
|Ex/Em Maxima:||Ex: 745nm|
|Storage:||Protect from light. Once reconstituted, store aliquoted at -20C for up to 3 months (avoid repeated freeze / thaw cycles). For long term storage, keep lyophilized at -20C for up to 12 months.|
Dissolve Neutrophil-Specific, NIR Fluorescent Imaging Agent in water or neutral buffer for a working solution concentration of 20uM. Inject mice with 50uL Working Solution per 10g body weight via intravenous injection.
- Inject mice at 2nmol per 20g of body weight.
- The half life of this compound is between 1.5 - 2hrs
- L. Xiao, Y. Zhang, M. D. Chordia, S. S. Berr, P. Pramoonjago, L. Pu, D. Pan. A novel near-infrared fluorescence imaging probe for in vivo neutrophil tracking. Mol Imaging. 2012 Sep-Oct;11(5):372-82.
- Xiao et al. Synthesis of the Cyanine 7 labeled neutrophil-specific agents for noninvasive near infrared fluorescence imaging. Bioorg. Med. Chem. Lett. 20 (2010) 35153517.
- Mott TM, Johnston RK, Vijayakumar S, Estes DM, Motamedi M, Sbrana E, Endsley JJ, and Torres A. Monitoring Therapeutic Treatments against Burkholderia Infections Using Imaging Techniques. Pathogens 2 (2013) 383-401. View Article
- Pletzer D, Mansour SC, Wuerth K, Rahanjam N, Hancock RE. New Mouse Model for Chronic Infections by Gram-Negative Bacteria Enabling the Study of Anti-Infective Efficacy and Host-Microbe Interactions. MBio. 2017 Feb 28;8(1). pii: e00140-17. doi: 10.1128/mBio.00140-17. PubMed PMID: 28246361; PubMed Central PMCID: PMC5347345. View Article
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