Anti-VSV-G [IE9F9] Antibody
This monoclonal antibody reacts with VSV-G protein from the Indiana serotype.
Highlights:
- Shows reactivity against VSV-G pseudotyped lentiviruses (see references below)
- Suitable for Western Blot, Flow Cytometry and Immunofluorescence applications
Recombinant versions available from our sister company, Absolute Antibody:
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma IE9F9
Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The G protein (glycoprotein) is located at the virion surface and is responsible for virus attachment and penetration. Additionally, many lentivrial vectors are pseudotyped with VSV-G from the Indiana serotype.
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
This monoclonal antibody reacts with VSV-G protein from the Indiana serotype.
Highlights:
- Shows reactivity against VSV-G pseudotyped lentiviruses (see references below)
- Suitable for Western Blot, Flow Cytometry and Immunofluorescence applications
Recombinant versions available from our sister company, Absolute Antibody:
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma IE9F9
Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The G protein (glycoprotein) is located at the virion surface and is responsible for virus attachment and penetration. Additionally, many lentivrial vectors are pseudotyped with VSV-G from the Indiana serotype.
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Antibody |
| Name: | Anti-VSV-G [IE9F9] |
| Antigen: | VSV-G |
| Accession ID: | P03522 |
| Host: | Mouse |
| Isotype: |
EB0012: IgG2a kappa Recombinant versions: see product name |
| Clonality: | Monoclonal |
| Clone Name: | Ie9F9 (I14) |
| Specificity: | VSV-Ind glycoprotein (G) protein |
| Reactivity: | Human |
| Immunogen: | VSV infection |
| Format: | Liquid |
| Purification Method: | Protein G purified |
| Buffer: |
EB0012: PBS, 0.05% (w/v) Sodium Azide Recombinant versions: PBS with 0.02% Proclin 301 |
| Tested Applications: | Western blot (1:1000) |
| Concentration: | 1mg/mL |
| Amount: | 100uL |
| Storage: | -20C (avoid repeated freeze / thaw cycles) |
| Shipped: | Cold packs |
Provider
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
References
- Vandepol SB, Lefrancois L, Holland JJ. Sequences of the Major Anitbody Binding Epitopes of the Indiana Serotype of Vesicular Stomatitis Virus. Virology 148: 312-325 (1986).
- Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982.
- Genevini P, Papiani G, Ruggiano A, Cantoni L, Navone F, Borgese N. Amyotrophic lateral sclerosis-linked mutant VAPB inclusions do not interfere with protein degradation pathways or intracellular transport in a cultured cell model. PLoS One. 2014 Nov 19;9(11):e113416. doi: 10.1371/journal.pone.0113416. View Article
- Yonemura Y, Li X, Müller K, Krämer A, Atigbire P, Mentrup T, Feuerhake T, Kroll T, Shomron O, Nohl R, Arndt HD, Hoischen C, Hemmerich P, Hirschberg K, Kaether C. Inhibition of cargo export at ER exit sites and the trans-Golgi network by the secretion inhibitor FLI-06. J Cell Sci. 2016 Oct 15;129(20):3868-3877. PubMed PMID: 27587840.View Article
- Fossati M, Colombo SF, Borgese N. A positive signal prevents secretory membrane cargo from recycling between the Golgi and the ER. EMBO J. 2014 Sep 17;33(18):2080-97. doi: 10.15252/embj.201488367. PubMed PMID: 25063674; PubMed Central PMCID: PMC4195774. View Article
- Tezuka K, Okuma K, Kuramitsu M, Matsuoka S, Tanaka R, Tanaka Y, Hamaguchi I. Control of HTLV-1 Infection by Eliminating Envelope Protein-Positive Cells with Recombinant Vesicular Stomatitis Viruses Encoding HTLV-1 Primary Receptor. J Virol. 2017 Dec 6. pii: JVI.01885-17. View Article
- Tam AB, Roberts LS, Chandra V, Rivera IG, Nomura DK, Forbes DJ, Niwa M. The UPR Activator ATF6 Responds to Proteotoxic and Lipotoxic Stress by Distinct Mechanisms. Dev Cell. 2018 Aug 6. View Article
- Munis AM, Tijani M, Hassall M, Mattiuzzo G, Collins MK, Takeuchi Y. Characterization of Antibody Interactions with the G Protein of Vesicular Stomatitis Virus Indiana Strain and Other Vesiculovirus G Proteins. J Virol. 2018 Nov 12;92(23). pii: e00900-18. View Article
- Johnson B, VanBlargan LA, Xu W, White JP, Shan C, Shi PY, Zhang R, Adhikari J, Gross ML, Leung DW, Diamond MS, Amarasinghe GK. Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability. Immunity. 2018 Mar 20;48(3):487-499.e5. View Article
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