Anti-VSV-G [8G5F11] Antibody

This monoclonal antibody reacts with VSV-G protein from the Indiana serotype.


  • Shows reactivity against VSV-G pseudotyped lentiviruses (see references below).

Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The G protein (glycoprotein) is located at the virion surface and is responsible for virus attachment and penetration. Additionally, many lentivrial vectors are pseudotyped with VSV-G from the Indiana serotype.

From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.

Catalog Number Product Size AVAILABILITY Price Qty
Anti-VSV-G [8G5F11] Antibody, 100 ug
100ug (1mg/mL) In stock
Price: $359.00
Product Type: Antibody
Name: anti-VSV-G (8G5F11)
Isotype: IgG2a kappa
Clonality: Monoclonal
Clone Name: 8G5FL11 (I1)
Specificity: VSV-Ind glycoprotein (G) protein
Immunogen: VSV infection
Host: Mouse
Format: Liquid
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: Western blot (1:1000), flow cytometry, immunofluorescence
Purity: Protein G purified
Concentration: 1mg/mL
Amount: 100uL
Storage: -20C (avoid repeated freeze / thaw cycles)
Shipped: Cold packs

BHK cells were infected with VSV and labeled with Anti-VSV-G (8G5F11) and JacksonLabs Dylight 549 goat anti-mouse secondary antibody. Cells were imaged at 20X using QImaging Camera (half of 14-bit range with < 1000 ms exposure). Cells were stained and imaged live. (contrast adjusted 8-bit image).

Image courtesy of KeraFAST Customer Jay Warrick, Ph.D., University of Wisconsin Madison, submitted November 16, 2011.

From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
  1. Gregory DA, Olinger GY, Lucas TM, Johnson MC. Diverse viral glycoproteins as well as CD4 co-package into the same human immunodeficiency virus (HIV-1) particles. Retrovirology. 2014 Apr 3;11(1):28.
  2. Kubo S, Mitani K. A New Hybrid System Capable of Efficient Lentiviral Vector Production and Stable Gene Transfer Mediated by a Single Helper-Dependent Adenoviral Vector. JVirol 77: 2964-2971, 2003.
  3. Kobayashi M, Iida A, Ueda Y, and Hasegawa M. Pseudotyped Lentivirus Vectors Derived from Simian Immunodeficiency Virus SIVagm with Envelope Glycoproteins from Paramyxovirus. JVirol 77: 2607-2614, 2003.
  4. Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. PNAS 90: 8033-8037, 1993.
  5. Vandepol SB, Lefrancois L, Holland JJ. Sequences of the Major Anitbody Binding Epitopes of the Indiana Serotype of Vesicular Stomatitis Virus. Virology 148: 312-325 (1986).
  6. Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982.
  7. Boso G, Somia NV. Characterization of resistance to rhabdovirus and retrovirus infection in a human myeloid cell line. PLoS One. 2015 Mar 26;10(3):e0121455. doi: 10.1371/journal.pone.0121455. View Article
  8. Zhang J, Reiling C, Reinecke JB, Prislan I, Marky LA, Sorgen PL, Naslavsky N, Caplan S. Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytictrafficking and retromer function. Traffic. 2012 May;13(5):745-57. View Article
  9. McNulty S, Flint M, Nichol ST, Spiropoulou CF. Host mTORC1 signaling regulatesandes virus replication. J Virol. 2013 Jan;87(2):912-22. View Article
  10. Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman BF, Fauci AS, Lusso P. The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent,broad-spectrum inhibitor of HIV-1. PLoS Pathog. 2013;9(12):e1003852. View Article
  11. Xing M, Peterman MC, Davis RL, Oegema K, Shiau AK, Field SJ. GOLPH3 drives cell migration by promoting Golgi reorientation and directional trafficking to the leading edge. Mol Biol Cell. 2016 Dec 1;27(24):3828-3840. PubMed PMID: 27708138; PubMed Central PMCID: PMC5170606.View Article
  12. Yonemura Y, Li X, Müller K, Krämer A, Atigbire P, Mentrup T, Feuerhake T, Kroll T, Shomron O, Nohl R, Arndt HD, Hoischen C, Hemmerich P, Hirschberg K, Kaether C. Inhibition of cargo export at ER exit sites and the trans-Golgi network by the secretion inhibitor FLI-06. J Cell Sci. 2016 Oct 15;129(20):3868-3877. PubMed PMID: 27587840. View Article
  13. Yuan WC, Lee YR, Lin SY, Chang LY, Tan YP, Hung CC, Kuo JC, Liu CH, Lin MY, Xu M, Chen ZJ, Chen RH. K33-Linked Polyubiquitination of Coronin 7 by Cul3-KLHL20 Ubiquitin E3 Ligase Regulates Protein Trafficking. Mol Cell. 2014 May 22;54(4):586-600. doi: 10.1016/j.molcel.2014.03.035. PubMed PMID: 24768539. View Article
  14. McNulty S, Flint M, Nichol ST, Spiropoulou CF. Host mTORC1 signaling regulates andes virus replication. J Virol. 2013 Jan;87(2):912-22. doi: 10.1128/JVI.02415-12. PubMed PMID: 23135723; PubMed Central PMCID: PMC3554081. View Article
  15. Ferlin A, Raux H, Baquero E, Lepault J, Gaudin Y. Characterization of pH-sensitive molecular switches that trigger the structural transition of vesicular stomatitis virus glycoprotein from the postfusion state toward the prefusion state. J Virol. 2014 Nov;88(22):13396-409. doi: 10.1128/JVI.01962-14. PubMed PMID: 25210175; PubMed Central PMCID: PMC4249061. View Article
  16. Rao M, Song W, Jiang A, Shyr Y, Lev S, Greenstein D, Brantley-Sieders D, Chen J. VAMP-associated protein B (VAPB) promotes breast tumor growth by modulation of Akt activity. PLoS One. 2012;7(10):e46281. doi: 10.1371/journal.pone.0046281. PubMed PMID: 23049696; PubMed Central PMCID: PMC3462209. (supplemental information) View Article
  17. Swick A, Baltes A, Yin J. Visualizing infection spread: dual-color fluorescent reporting of virus-host interactions. Biotechnol Bioeng. 2014 Jun;111(6):1200-9. doi: 10.1002/bit.25170. PubMed PMID: 24338628; PubMed Central PMCID: PMC4004699. View Article
  18. Baquero E, Albertini AA, Raux H, Abou-Hamdan A, Boeri-Erba E, Ouldali M, Buonocore L, Rose JK, Lepault J, Bressanelli S, Gaudin Y. Structural intermediates in the fusion-associated transition of vesiculovirus glycoprotein. EMBO J. 2017 Mar 1;36(5):679-692. doi: 10.15252/embj.201694565. PubMed PMID: 28188244; PubMed Central PMCID: PMC5331758. View Article
  19. Sakata M, Tani H, Anraku M, Kataoka M, Nagata N, Seki F, Tahara M, Otsuki N, Okamoto K, Takeda M, Mori Y. Analysis of VSV pseudotype virus infection mediated by rubella virus envelope proteins. Sci Rep. 2017 Sep 14;7(1):11607. View Article
  20. Anandi L, Chakravarty V, Ashiq KA, Bodakuntla S, Lahiri M. DNA-PK plays a central role in transformation of breast epithelial cells following alkylation damage. J Cell Sci. 2017 Sep 18. pii: jcs.203034. View Article
  21. Milani M, Annoni A, Bartolaccini S, Biffi M, Russo F, Di Tomaso T, Raimondi A, Lengler J, Holmes MC, Scheiflinger F, Lombardo A, Cantore A, Naldini L. Genome editing for scalable production of alloantigen-free lentiviral vectors for in vivo gene therapy. EMBO Mol Med. 2017 Nov;9(11):1558-1573. View Article
  22. Tezuka K, Okuma K, Kuramitsu M, Matsuoka S, Tanaka R, Tanaka Y, Hamaguchi I. Control of HTLV-1 Infection by Eliminating Envelope Protein-Positive Cells with Recombinant Vesicular Stomatitis Viruses Encoding HTLV-1 Primary Receptor. J Virol. 2017 Dec 6. pii: JVI.01885-17. View Article

If you publish research with this product, please let us know so we can cite your paper.