Anti-Influenza A, Non-Structural Protein 1 (NS1) Antibodies
These mouse IgG monoclonal antibodies were generated against affinity-purified non-structural protein (NS1) and recognize a broad range of influenza virus NS1 proteins.
Highlights:
- Broadly cross reactive & highly NS1 specific - Shown to recognize multiple H1N1 and H3N2 subtypes
- Predicted to react with >90% of currently known H1-H16 NS1 types, based on epitope mapping and sequence comparisons
- Potential diagnostic tool for tracking productive IAV replicates & characterizing NS1 structure & function
- Nuclear & cytoplasmic detection as early as 5 hrs post-infection
- Suitable for Western Blot, Immunofluorescence Microscopy, and Immunoprecipitation applications (other applications not yet tested)
Influenza A viruses (IAV) are negative-sense, single-stranded, segmented RNA viruses. The NS1 influenza protein (NS1) is a viral nonstructural protein encoded by the NS gene segments of type A, B and C influenza viruses. NS1 inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. NS1 prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7.
From the laboratory of Kevin M. Coombs, PhD, University of Manitoba.
These mouse IgG monoclonal antibodies were generated against affinity-purified non-structural protein (NS1) and recognize a broad range of influenza virus NS1 proteins.
Highlights:
- Broadly cross reactive & highly NS1 specific - Shown to recognize multiple H1N1 and H3N2 subtypes
- Predicted to react with >90% of currently known H1-H16 NS1 types, based on epitope mapping and sequence comparisons
- Potential diagnostic tool for tracking productive IAV replicates & characterizing NS1 structure & function
- Nuclear & cytoplasmic detection as early as 5 hrs post-infection
- Suitable for Western Blot, Immunofluorescence Microscopy, and Immunoprecipitation applications (other applications not yet tested)
Influenza A viruses (IAV) are negative-sense, single-stranded, segmented RNA viruses. The NS1 influenza protein (NS1) is a viral nonstructural protein encoded by the NS gene segments of type A, B and C influenza viruses. NS1 inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. NS1 prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7.
From the laboratory of Kevin M. Coombs, PhD, University of Manitoba.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Influenza virus NS1 protein |
| Accession ID: | P03495 |
| Molecular Weight: | 26 kDa |
| Isotype: | IgG2a ? |
| Clone Name: | 5D6 |
| Reactivity: | Broadly cross-reactive & highly NS1 specific. Reactive against multiple H1N1 and H3N2 subtypes, and, based on epitope mapping and sequence comparisons, predicted to react with > 90% of currently known H1-H16 NS1 types. |
| Immunogen: | Affinity-purified His6-tagged NS1 from A/Hong Kong/1/1968 (H3N2) |
| Species Immunized: | Mouse |
| Epitope: | See Table |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | Western Blot, Immunofluorescence Microscopy, and Immunoprecipitation applications (other applications not yet tested) |
| Storage: | -20C |
| Shipped: | Cold packs |
| Clone | Western Blot | Immunofluorescence | Epitope |
| 5D6 | Strong Reactivity | Weak Reactivity | 12-DCFLWHVR-19 |
| 10C7 | Strong Reactivity | Strong Reactivity | 12-DCFLWHVR-19 |
| 7D11 | Strong Reactivity | Medium Reactivity | 29-DAPFLDR-35 |
| 5B10 | Weak Reactivity | Medium Reactivity | 138-FDRLETLILL-147 |
| 3F5 | Strong Reactivity | Strong Reactivity | 161-PLPSLPGH-169 |
| 5F4 | Medium Reactivity | Strong Reactivity | 161-PLPSLPGH-169 |
| 13D8 | Strong Reactivity | Strong Reactivity | 161-PLPSLPGH-169 |
| 8C7 | Medium Reactivity | Strong Reactivity | 161-PLPSLPGH-169 |
| 4E10 | Medium Reactivity | No Reactivity | 211-RPPLT-215 |
- Rahim MN, Selman M, Sauder PJ, Forbes NE, Stecho W, Xu W, Lebar M, Brown EG, Coombs KM. Generation and characterization of a new panel of broadly reactive anti-NS1 mAbs for detection of influenza A virus. J Gen Virol. 2013 Mar;94(Pt3):593-605
- Chaimayo C, Dunagan M, Hayashi T, Santoso N, Takimoto T. Specificity and functional interplay between influenza virus PA-X and NS1 shutoff activity. PLoS Pathog. 2018 Nov 29. View Article
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