Anti-Alpha-Spectrin, N-terminal 80 kDa Domain Antibody
This rabbit IgG polyclonal antibody was generated against human red blood cell a-spectrin N terminal 1/4th and is specific for human red blood cell a-spectrin N terminal 1/4th of the protein.
Highlights:
- Reacts with human red blood cell a-spectrin N terminal 1/4th of the protein
- Recommended for Western Blot applications
Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane in eukaryotic cells along with actin. Spectrin plays a role in cell shape control, arrangement of transmembrane proteins, and organelle positioning. Specifically, Alpha-spectrin is a tetramer and mutations in alpha-spectrin can result in a variety of hereditary red blood cell disorders (e.g. spherocytic hemolytic anemia including elliptocytosis type 2, pyropoikilocytosis).
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
This rabbit IgG polyclonal antibody was generated against human red blood cell a-spectrin N terminal 1/4th and is specific for human red blood cell a-spectrin N terminal 1/4th of the protein.
Highlights:
- Reacts with human red blood cell a-spectrin N terminal 1/4th of the protein
- Recommended for Western Blot applications
Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane in eukaryotic cells along with actin. Spectrin plays a role in cell shape control, arrangement of transmembrane proteins, and organelle positioning. Specifically, Alpha-spectrin is a tetramer and mutations in alpha-spectrin can result in a variety of hereditary red blood cell disorders (e.g. spherocytic hemolytic anemia including elliptocytosis type 2, pyropoikilocytosis).
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
| Product Type: | Antibody |
| Alternative Name(s): | Erythroid alpha-spectrin,Spectrin alpha chain, erythrocytic 1 |
| Antigen: | Human Red Blood Cell a-Spectrin N terminal 1/4th of the protein |
| Accession ID: | P02549 |
| Molecular Weight: | 280 kDa |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Human |
| Immunogen: | Alpha-spectrin 80kDa domain (approximately residues 7-601) |
| Species Immunized: | Rabbit |
| Tested Applications: | Western Blot (1: 500,000; 5sec exposure) |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Western Blot
Lane 1, Human Red Cell Membrane; Lane 2, Human Melanoma (1205 LU); Lane 3, Human Ovarian Cancer (RMG1); Lane 4, Human Ovarian Cancer (OV90). Gel loads: Lane 1, 5mg; Lanes 2-4, 25mg. Antibody dilution: 1:500,000, 5sec exposure.
- Speicher DW, DeSilva TM, Speicher KD, Ursitti JA, Hembach P, Weglarz L. Location of the human red cell spectrin tetramer binding site and detection of a related "closed" hairpin loop dimer using proteolytic footprinting. J Biol Chem. 1993 Feb 25;268(6):4227-35.
- Zimmerman UJ, Speicher DW, Fisher AB. Secretagogue-induced proteolysis of lungspectrin in alveolar epithelial type II cells. Biochim Biophys Acta. 1992 Oct27;1137(2):127-34.
- Speicher DW, DeSilva TM, Speicher KD, Ursitti JA, Hembach P, Weglarz L.Location of the human red cell spectrin tetramer binding site and detection of a related "closed" hairpin loop dimer using proteolytic footprinting. J Biol Chem. 1993 Feb 25;268(6):4227-35.
- Kotula L, DeSilva TM, Speicher DW, Curtis PJ.Functional characterization of recombinant human red cell alpha-spectrin polypeptides containing the tetramer binding site.J Biol Chem. 1993 Jul 15;268(20):14788-93.
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