Anti-Survival Motor Neuron (SMN) [4F11] Antibody
![Anti-Survival Motor Neuron (SMN) [4F11] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1140_2001202001263511390.jpg "Anti-Survival Motor Neuron (SMN) [4F11] Antibody")
This mouse monoclonal [4F11] antibody was generated against recombinant Survival Motor Neuron (SMN) protein and is specific for SMN exon 4 of human SMN.
Highlights:
- Reacts with human exon 4 of SMN
- Suitable for Western Blot and Immunofluorescence applications
The Survival motor neuron (SMN) protein complex is an essential component of the spliceosomal machinery. Defects in survival motor neuron protein 1 (SMN1), an intrigal component of the complex, lead to the autosomal recessive disorder, spinal muscular atrophy (SMA). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy.
From the laboratory of Christian L. Lorson, PhD, University of Missouri - Columbia.
Part of The Investigator's Annexe program.
This mouse monoclonal [4F11] antibody was generated against recombinant Survival Motor Neuron (SMN) protein and is specific for SMN exon 4 of human SMN.
Highlights:
- Reacts with human exon 4 of SMN
- Suitable for Western Blot and Immunofluorescence applications
The Survival motor neuron (SMN) protein complex is an essential component of the spliceosomal machinery. Defects in survival motor neuron protein 1 (SMN1), an intrigal component of the complex, lead to the autosomal recessive disorder, spinal muscular atrophy (SMA). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy.
From the laboratory of Christian L. Lorson, PhD, University of Missouri - Columbia.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | Survival Motor Neuron (SMN) |
| Accession ID: | P97801 |
| Molecular Weight: | 38 kDa |
| Clonality: | Monoclonal |
| Clone Name: | 4F11 |
| Reactivity: | Human |
| Immunogen: | Recombinant SMN protein |
| Species Immunized: | Mouse |
| Epitope: | SMN exon 4 |
| Purification Method: | Protein G |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB: 1:1000; IF: 1:1000 |
| Storage: | -20C |
| Shipped: | Cold packs |
Epitope Mapping by Western Blot
GST-tagged SMN exons were individually expressed in bacterial cells. Immunoblots were performed against the GST tag, the blot was then stripped and reprobed with anti-SMN 4F11. The 4F11 maps to exon 4 of SMN.
Adapted from: Mattis V. et al., J Neurosci Methods. 2008 Oct 30;175(1):36-43.- Coady TH, Baughan TD, Shababi M, Passini MA, Lorson CL. Development of a single vector system that enhances trans-splicing of SMN2 transcripts. PLoS One. 2008;3(10):e3468.
- Baughan T, Shababi M, Coady TH, Dickson AM, Tullis GE, Lorson CL. Stimulating full-length SMN2 expression by delivering bifunctional RNAs via a viral vector. Mol Ther. 2006 Jul;14(1):54-62.
- Mattis VB, Butchbach ME, Lorson CL. Detection of human survival motor neuron (SMN) protein in mice containing the SMN2 transgene: applicability to preclinical therapy development for spinal muscular atrophy. J Neurosci Methods. 2008 Oct 30;175(1):36-43.
- Wolstencroft EC, Mattis V, Bajer AA, Young PJ, Lorson CL. A non-sequence-specific requirement for SMN protein activity: the role of aminoglycosides in inducing elevated SMN protein levels. Hum Mol Genet. 2005 May 1;14(9):1199-210.
- Mattis VB, Ebert AD, Fosso MY, Chang CW, Lorson CL. Delivery of a read-through inducing compound, TC007, lessens the severity of a spinal muscular atrophy animal model. Hum Mol Genet. 2009 Oct 15;18(20):3906-13.
- Mattis VB, Bowerman M, Kothary R, Lorson CL. A SMNDelta7 read-through product confers functionality to the SMNDelta7 protein. Neurosci Lett. 2008 Sep 5;442(1):54-8.
- Wolstencroft EC1 Mattis V, Bajer AA, Young PJ, Lorson CL. A non-sequence-specific requirement for SMN protein activity: the role of aminoglycosides in inducing elevated SMN protein levels. Hum Mol Genet. 2005 May 1;14(9):1199-210.
- Mattis VB, Butchbach ME, Lorson CL. Detection of human survival motor neuron (SMN) protein in mice containing the SMN2 transgene: applicability to preclinical therapy development for spinal muscular atrophy. J Neurosci Methods. 2008 Oct 30;175(1):36-43.
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