Anti-dsRNA [rJ2] Antibody

This mouse IgG2a monoclonal antibody [rJ2], recognizes double stranded RNA (dsRNA) of greater than 40 bp in length which is generated during the replication of positive sense genome viruses.

Highlights:

  • Reacts with dsRNA of positive sense genome viruses
  • Suitable for ELISA, Immunoflourescence, Immunohistochemistry, Immuno-EM and Affinity Purification applications

rJ2 antibody can be used to detect dsRNA intermediates of viruses as diverse as Zika virus, Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples.

For more information on how this antibody reacts with Zika virus see a blog post from ASM: Zika Virus Inside Cells

From the laboratory of Abraham L. Brass, MD, PhD, University of Massachusetts Medical School.

Catalog Number Product Size AVAILABILITY Price Qty
ES2001
Anti-dsRNA [rJ2] Antibody, (supernatant), 4mL
Supernatant, 4mL (2x2mL) In stock
Price: $376.00
Specifications
Antigen: double stranded RNA produced by positive sense genome viruses
Molecular Weight: dsRNA > 40 bp in length
Isotype: IgG2a, kappa light chain
Clonality: Monoclonal
Clone Name: Recombinant J2 (rJ2)
Reactivity: Positive sense genome viruses
Immunogen: dsRNA
Species Immunized: Mouse
Epitope: dsRNA from positive sense genome virus replication
Buffer: Culture medium, DMEM and 5% FBS
Tested Applications: IF (1:60), IHC, ELISA, immuno-EM, Affinity purification
Storage: -80C
Shipped: Dry ice
Data

Zika Virus Infecting Cells

Anti-dsRNA [rJ2] Antibody used at 1:60 to detect Zika virus (green) infection of cultured human cells. Zika virus levels are lower (top right panel), as UMMS researchers decreased the levels of the EMC1 protein in the human cells using siRNA. NT= non-targeting negative control siRNA. Cell nuclei are shown in blue below

Adapted from: Savidis G, et al. Cell Reports June 21 2016

Provider
From the laboratory of Abraham L. Brass, MD, PhD, University of Massachusetts Medical School.
References
  1. Savidis G, Perreira JM, Portmann JM, Meraner P, Guo Z, Green S, Brass AL. The IFITMs Inhibit Zika Virus Replication. Cell Rep. 2016 Jun 14;15(11):2323-30. View Article
  2. George Savidis, William M. Mcdougall, Paul Meraner, Jill M. Perreira, Jocelyn M. Portmann, Gaia Trincucci, Sinu P. John, Aaron M. Aker, Nicholas Renzette, Douglas R. Robbins, Zhiru Guo, Sharone Green, Timothy F. Kowalik, Abraham L. Brass. Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. Cell Reports, June 2016 DOI: 10.1016/j.celrep.2016.06.028

If you publish research with this product, please let us know so we can cite your paper.

 
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