Anti-dsRNA [rJ2] Antibody
This mouse IgG2a monoclonal antibody [rJ2], recognizes double stranded RNA (dsRNA) of greater than 40 bp in length which is generated during the replication of positive sense genome viruses.
Highlights:
- Reacts with dsRNA of positive sense genome viruses
- Suitable for Immunoflourescence, Immunohistochemistry, Immuno-EM and Affinity Purification applications
Recombinant versions available from our sister company, Absolute Antibody:
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma J2
rJ2 antibody can be used to detect dsRNA intermediates of viruses as diverse as Zika virus, Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples.
For more information on how this antibody reacts with Zika virus see a blog post from ASM: Zika Virus Inside Cells
From the laboratory of Abraham L. Brass, MD, PhD, University of Massachusetts Medical School.
This mouse IgG2a monoclonal antibody [rJ2], recognizes double stranded RNA (dsRNA) of greater than 40 bp in length which is generated during the replication of positive sense genome viruses.
Highlights:
- Reacts with dsRNA of positive sense genome viruses
- Suitable for Immunoflourescence, Immunohistochemistry, Immuno-EM and Affinity Purification applications
Recombinant versions available from our sister company, Absolute Antibody:
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma J2
rJ2 antibody can be used to detect dsRNA intermediates of viruses as diverse as Zika virus, Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples.
For more information on how this antibody reacts with Zika virus see a blog post from ASM: Zika Virus Inside Cells
From the laboratory of Abraham L. Brass, MD, PhD, University of Massachusetts Medical School.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Name: | Anti-dsRNA [rJ2] Antibody |
| Antigen: | double stranded RNA produced by positive sense genome viruses |
| Molecular Weight: | dsRNA > 40 bp in length |
| Isotype: |
ES2001: IgG2a kappa Recombinant versions: see product name |
| Clonality: | Monoclonal |
| Fusion Tag(s): | His-Tagged |
| Clone Name: | Recombinant J2 (rJ2) |
| Reactivity: | Positive sense genome viruses |
| Specificity: | Positive sense genome viruses |
| Immunogen: | dsRNA |
| Species Immunized: | Mouse |
| Epitope: | dsRNA from positive sense genome virus replication |
| Buffer: |
ES2001: Culture medium, DMEM and 5% FBS Recombinant versions: PBS with 0.02% Proclin 300 |
| Tested Applications: | IF (1:60), IHC, immuno-EM, Affinity purification |
| Storage: |
ES2001: -80C Recombinant versions: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C |
| Shipped: |
ES2001: Dry ice Recombinant versions: Cold packs |
Zika Virus Infecting Cells
Anti-dsRNA [rJ2] Antibody used at 1:60 to detect Zika virus (green) infection of cultured human cells. Zika virus levels are lower (top right panel), as UMMS researchers decreased the levels of the EMC1 protein in the human cells using siRNA. NT= non-targeting negative control siRNA. Cell nuclei are shown in blue below
Adapted from: Savidis G, et al. Cell Reports June 21 2016
- Savidis G, Perreira JM, Portmann JM, Meraner P, Guo Z, Green S, Brass AL. The IFITMs Inhibit Zika Virus Replication. Cell Rep. 2016 Jun 14;15(11):2323-30. View Article
- George Savidis, William M. Mcdougall, Paul Meraner, Jill M. Perreira, Jocelyn M. Portmann, Gaia Trincucci, Sinu P. John, Aaron M. Aker, Nicholas Renzette, Douglas R. Robbins, Zhiru Guo, Sharone Green, Timothy F. Kowalik, Abraham L. Brass. Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. Cell Reports, June 2016 DOI: 10.1016/j.celrep.2016.06.028
- Samuelsen S, Jørgensen CD, Mellins ED, Torok KS, Astakhova K. Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens. PLoS One. 2018 Apr 11;13(4):e0195381. View Article
- Shelkovnikova TA, Kukharsky MS, An H, Dimasi P, Alexeeva S, Shabir O, Heath PR, Buchman VL. Protective paraspeckle hyper-assembly downstream of TDP-43 loss of function in amyotrophic lateral sclerosis. Mol Neurodegener. 2018 Jun 1;13(1):30. View Article
- Jena KK, Mehto S, Nath P, Chauhan NR, Sahu R, Dhar K, Das SK, Kolapalli SP, Murmu KC, Jain A, Krishna S, Sahoo BS, Chattopadhyay S, Rusten TE, Prasad P, Chauhan S, Chauhan S. Autoimmunity gene IRGM suppresses cGAS-STING and RIG-I-MAVS signaling to control interferon response. EMBO Rep. 2020 Sep 3;21(9):e50051. View article
- Yin X, Riva L, Pu Y, Martin-Sancho L, Kanamune J, Yamamoto Y, Sakai K, Gotoh S, Miorin L, De Jesus PD, Yang CC, Herbert KM, Yoh S, Hultquist JF, García-Sastre A, Chanda SK. MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells. Cell Rep. 2021 Jan 12;34(2):108628. View article
If you publish research with this product, please let us know so we can cite your paper.
