|Name:||ALKO (astrocyte LKB1 knock out)|
|Cell Type:||Transformed mouse astrocyte|
|Subculturing:||Standard trypsinization followed by 1:10 dilution|
|Growth Conditions:||DMEM 10% FCS A/A|
|Cryopreservation:||10% DMSO in FBS|
Mouse ALKO cells (astrocyte LKB1 knock out) were prepared by treating astrocytes harboring a floxed version of the LKB1 gene with Adeno Associated Virus expressing the bacterial Cre-recombinase. After several passages, the resulting cells show anchorage independent growth, have a doubling time of less than 24 hr, lose expression of the astrocyte marker GFAP (Glial fibrillary acidic protein), and gain expression of stem cell markers including Sox2 and Oct4.
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