Anti-Xyloglucan [CCRC-M1] Antibody (supernatant)
This mouse IgG monoclonal (CCRC-M1) was generated against Rhamnogalacturonan I/MeBSA complex (Non-covalent) and recognizes the alpha-Fuc-(1,2)-beta-Gal glycan group of Fucosylated Xyloglucan.
Highlights:
- Reacts with dicot plants (sycamores) alpha-Fuc-(1,2)-beta-Gal glycan group of Fucosylated Xyloglucan
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Xyloglucan is a hemiceullose or polysaccharide that is found in the primary cell wall of all vascular plants. Xyloglucan binds to the surface of cellulose microfibrils and may link them together.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
This mouse IgG monoclonal (CCRC-M1) was generated against Rhamnogalacturonan I/MeBSA complex (Non-covalent) and recognizes the alpha-Fuc-(1,2)-beta-Gal glycan group of Fucosylated Xyloglucan.
Highlights:
- Reacts with dicot plants (sycamores) alpha-Fuc-(1,2)-beta-Gal glycan group of Fucosylated Xyloglucan
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Xyloglucan is a hemiceullose or polysaccharide that is found in the primary cell wall of all vascular plants. Xyloglucan binds to the surface of cellulose microfibrils and may link them together.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
| Product Type: | Antibody |
| Accession ID: | CCRC M001 |
| Antigen: | Xyloglucan, Rhamnogalacturonan I of the Sycamore maple (Acer pseudoplatanus) |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | CCRC M1 |
| Reactivity: | Dicot plants, especially sycamore |
| Immunogen: | Rhamnogalacturonan I/MeBSA complex (Non-covalent) |
| Species Immunized: | Mouse |
| Epitope: | alpha-Fuc-(1,2)-beta-Gal |
| Buffer: | Cell culture supernatant, Use undiluted or at 1:10 dilution |
| Tested Applications: | ELISA, Immunolabeling, IF |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
The epitope recognized by CCRC-M1 is most prevalent in xyloglucans from many dicots, and is present to a small extent in rhamnogalacturonan I from some dicots.
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
- DeMartini, JD, Pattathil, S, Avci, U, Szekalski, K, Mazumder, K, Hahn, M.G., Wyman, CE: Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production. Energy Environ. Sci., 2011, 4, 4332-4339.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1,2)-linked fucosyl-containing epitope. Plant Physiol. 104:699-710.
- G. Freshour, R. P. Clay, M. S. Fuller, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1996) Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol. 110:1413-1429.
- G. Freshour, C. P. Bonin, W.-D. Reiter, P. Albersheim, A. G. Darvill, and M. G. Hahn. (2003) Distribution of fucose-containing xyloglucans in cell walls of the mur1 mutant of Arabidopsis thaliana. Plant Physiol. 131:1602-1612.
- Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- A.P. de Souza, D.C.C. Leite, S. Pattahil, M.G. Hahn, M.S. Buckeridge. 2013. Composition and structure of sugarcane cell wall polysaccharides: Implications for second generation bioethanol production. Bioenergy Research 6: 564-579.
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive tool kit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
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