Anti-Polycystin-1 [E8-8C3C10] Antibody

This mouse IgG1 monoclonal antibody [E8-8C3C10] was generated against 6xHis-tagged fusion protein and recognizes the third extracellular loop (i.e. directed to the C-terminal cleavage fragment) of polycystin-1 (PC1).

Highlights

  • Reacts with human and mouse Polycystin-1
  • Suitable for Western Blot and Immunoprecipitation applications

Polycystin-1 (PC1) is the gene product of PKD1 that is mutated in human autosomal dominant polycystic Kidney disease. It is required for tubular morphogenesis and maintenance of the kidney and other organs. PC1 is a 11-transmembrane glycoprotein that is cic-autoproteolytically cleaved at juxtamembrane extracellular GPS/GAIN in vivo, resulting a N-terminal cleavage fragment and a C-terminal cleavage fragment in the kidney and other tissues.

From the laboratories of Feng Qian, PhD, and Terry J. Watnick, MD, University of Maryland, Baltimore

Catalog Number Product Size AVAILABILITY Price Qty
EMD303
Anti-Polycystin-1 [E8-8C3C10] Antibody, 100ug
100ug In stock
Price: $333.00
Specifications
Antigen: Polycystin-1
Accession ID: NP_000287.3
Isotype: IgG1
Clonality: Monoclonal
Clone Name: E8-8C3C10
Reactivity: Human and mouse
Immunogen: 6xHis-tagged fusion protein
Species Immunized: Mouse
Epitope: Third extracellular loop of mouse PC1
Purification Method: Protein G Affinity
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1ug/mL) and IP(0.1-2ug per 2mg of protein)
Storage: -20C
Shipped: Cold packs
Data

Western Blot

Immunoblot detection of recombinant human or mouse PC1 by E8. E8 can detect full length (FL)PC1 and the C-terminal fragment (CTF).

Provider
From the laboratories of Feng Qian, PhD, and Terry J. Watnick, MD, University of Maryland, Baltimore
References
  1. Yu S, Hackmann K, Gao J, He X, Piontek K, García-González MA, Menezes LF, Xu H, Germino GG, Zuo J, Qian F. Essential role of cleavage of Polycystin-1 at G protein-coupled receptor proteolytic site for kidney tubular structure. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18688-93.

If you publish research with this product, please let us know so we can cite your paper.

 
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