Anti-Polycystin-1 [E8-8C3C10] Antibody
![Anti-Polycystin-1 [E8-8C3C10] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1019_2001202001263110180.jpg "Anti-Polycystin-1 [E8-8C3C10] Antibody")
This rat IgG1 monoclonal antibody [E8-8C3C10] was generated against 6xHis-tagged fusion protein and recognizes the third extracellular loop (i.e. directed to the C-terminal cleavage fragment) of polycystin-1 (PC1).
Highlights
- Reacts with human and mouse Polycystin-1
- Suitable for Western Blot and Immunoprecipitation applications
Polycystin-1 (PC1) is the gene product of PKD1 that is mutated in human autosomal dominant polycystic Kidney disease. It is required for tubular morphogenesis and maintenance of the kidney and other organs. PC1 is a 11-transmembrane glycoprotein that is cic-autoproteolytically cleaved at juxtamembrane extracellular GPS/GAIN in vivo, resulting a N-terminal cleavage fragment and a C-terminal cleavage fragment in the kidney and other tissues.
From the laboratories of Feng Qian, PhD, and Terry J. Watnick, MD, University of Maryland, Baltimore
This rat IgG1 monoclonal antibody [E8-8C3C10] was generated against 6xHis-tagged fusion protein and recognizes the third extracellular loop (i.e. directed to the C-terminal cleavage fragment) of polycystin-1 (PC1).
Highlights
- Reacts with human and mouse Polycystin-1
- Suitable for Western Blot and Immunoprecipitation applications
Polycystin-1 (PC1) is the gene product of PKD1 that is mutated in human autosomal dominant polycystic Kidney disease. It is required for tubular morphogenesis and maintenance of the kidney and other organs. PC1 is a 11-transmembrane glycoprotein that is cic-autoproteolytically cleaved at juxtamembrane extracellular GPS/GAIN in vivo, resulting a N-terminal cleavage fragment and a C-terminal cleavage fragment in the kidney and other tissues.
From the laboratories of Feng Qian, PhD, and Terry J. Watnick, MD, University of Maryland, Baltimore
| Product Type: | Antibody |
| Antigen: | Polycystin-1 |
| Accession ID: | P98161, NP_000287.3 |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | E8-8C3C10 |
| Reactivity: | Human and mouse |
| Immunogen: | 6xHis-tagged fusion protein |
| Species Immunized: | Rat |
| Epitope: | Third extracellular loop of mouse PC1 |
| Purification Method: | Protein G Affinity |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB (1ug/mL) and IP(0.1-2ug per 2mg of protein) |
| Storage: | -20C |
| Shipped: | Cold packs |
Western Blot
Immunoblot detection of recombinant human or mouse PC1 by E8. E8 can detect full length (FL)PC1 and the C-terminal fragment (CTF).
- Yu S, Hackmann K, Gao J, He X, Piontek K, García-González MA, Menezes LF, Xu H, Germino GG, Zuo J, Qian F. Essential role of cleavage of Polycystin-1 at G protein-coupled receptor proteolytic site for kidney tubular structure. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18688-93.
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