Anti-Polycystin-1 [E3] Antibody

This mouse IgG1 monoclonal antibody [E3] was generated against 6xHis-tagged fusion protein and recognizes the C-lectin domain of human Polycystin-1 (PC1) (i.e. directed to N-terminal cleavage fragment).

Highlights

  • Reacts with human and mouse Polycystin-1
  • Suitable for Western Blot, Immunoprecipitation and Immunofluorescence applications

Polycystin-1 (PC1) is the gene product of PKD1 that is mutated in human autosomal dominant polycystic Kidney disease. It is required for tubular morphogenesis and maintenance of the kidney and other organs. PC1 is a 11-transmembrane glycoprotein that is cic-autoproteolytically cleaved at juxtamembrane extracellular GPS/GAIN in vivo, resulting a N-terminal cleavage fragment and a C-terminal cleavage fragment in the kidney and other tissues.

From the laboratories of Feng Qian, PhD, and Terry J. Watnick, MD, University of Maryland, Baltimore

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EMD301
Anti-Polycystin-1 [E3] Antibody, 100ug
100ug In stock
Price: $333.00
Specifications
Antigen: Polycystin-1
Accession ID: NP_000287.3
Isotype: IgG1
Clonality: Monoclonal
Clone Name: E3
Reactivity: Human and mouse
Immunogen: 6xHis-tagged fusion protein
Species Immunized: Mouse
Epitope: C-lectin domain of human PC1
Purification Method: Protein G Affinity
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1:200-500), IP(0.1-2ug per 2mg of protein), IF (1:50)
Storage: -20C
Shipped: Cold packs
Data

Immunofluorescence

Immunofluorescence of detection of endogenous polycystin-1 in the cilia of mouse collecting ducts cells by E3.

Immunoprecipitation

Immunoprecipitation of endogenous polycystin-1 mouse lung tissue lysates E4 or 7E12. Immunoprecipitated PC1 is detected by immunoblot by 7E12 or anti-cc (directed against the c-terminal tail).

Provider
From the laboratories of Feng Qian, PhD, and Terry J. Watnick, MD, University of Maryland, Baltimore
References
  1. Yu S, Hackmann K, Gao J, He X, Piontek K, García-González MA, Menezes LF, Xu H, Germino GG, Zuo J, Qian F. Essential role of cleavage of Polycystin-1 at G protein-coupled receptor proteolytic site for kidney tubular structure. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18688-93.

If you publish research with this product, please let us know so we can cite your paper.

 
Loading...
Loading...