|Name:||Human Mast Cell Line (LUVA)|
|Cell Type:||Mast cell|
|Morphology:||Suspension and adherent|
|Subculturing:||Cells can be split 1:2-1:5 depending on how often cells are needed for experiments.|
|Growth Conditions:||StemPro-34 SFM (500mL), L-glutamine (2mM final), 5mL Pen Strep (10,000 U/mL), 1mL Primocin (50mg)|
|Cryopreservation:||pZerve cryopreservative (Protide Pharmaceuticals)|
|Source:||Blood CD34+ derived|
A difference has been noted in how the cells are behaving that is different from the original publication. In the article, LUVA cells had high levels of expression of FceRI, however levels of around 5% by cell surface staining are currently being observed. Higher levels with intracellular staining are seen and very high mRNA expression for FceRI is detected. The reason for this difference is not clear.
- Laidlaw, T.M., Steinke, J.W., Tiñana, A.M., Xing, W.,Lam, B.K., Paruchuri, S., Boyce, J.A., and Borish, L. Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional FcεRI. Journal of Allergy and Clinical Immunology 127:815-822.e5 (2011)
- Kirshenbaum AS, Goff JP, Semere T, Foster B, Scott, LM and Metcalfe DD Demonstration that human mast cells arise from a progenitor cell population that is CD34+, c-kit+, and expresses aminopeptidase N (CD13) Blood 1999; 94,7: 2333-2342
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