Anti-LRP6 [1C10] Antibody
![Anti-LRP6 [1C10] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/831_200120200126238300.jpg "Anti-LRP6 [1C10] Antibody")
This IgG1 mouse mononoclonal antibody was generated against the cytoplasmic domain of human LRP6 and is reactive against human, mouse, and rat LRP6.
Low density lipoprotein receptor-related protein 6 (LRP6) is a member of the low density lipoprotein receptor gene family (LDLR). LRP6 a cell surface protein, plays a critical role in embryonic development by functioning as a co-receptor specifically for the ß-catenin Wnt signaling pathway. LRP6 is highly homologous and presents itself as a dimer in the cell membrane until it is phosphlorylated by Wnt. When LRP6 binds to Wnt, it phosphorylates Wnt at multiple sites, which results in the recruitment of the scaffolding protein Axin to the cell membrane and ß catenin activation. LRP6 is the receptor for a novel Wnt antaganoist, DKK1. LRP6 is associated with autosomal dominant coronary artery disease type 2 and bone formation, as well as many human malignancies.
From the laboratory of Stuart A. Aaronson, MD, Icahn School of Medicine at Mount Sinai.
Part of The Investigator's Annexe program.
This IgG1 mouse mononoclonal antibody was generated against the cytoplasmic domain of human LRP6 and is reactive against human, mouse, and rat LRP6.
Low density lipoprotein receptor-related protein 6 (LRP6) is a member of the low density lipoprotein receptor gene family (LDLR). LRP6 a cell surface protein, plays a critical role in embryonic development by functioning as a co-receptor specifically for the ß-catenin Wnt signaling pathway. LRP6 is highly homologous and presents itself as a dimer in the cell membrane until it is phosphlorylated by Wnt. When LRP6 binds to Wnt, it phosphorylates Wnt at multiple sites, which results in the recruitment of the scaffolding protein Axin to the cell membrane and ß catenin activation. LRP6 is the receptor for a novel Wnt antaganoist, DKK1. LRP6 is associated with autosomal dominant coronary artery disease type 2 and bone formation, as well as many human malignancies.
From the laboratory of Stuart A. Aaronson, MD, Icahn School of Medicine at Mount Sinai.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Name: | Anti-LRP6 [1C10] Antibody |
| Accession ID: | O75581, AAI43726.1 |
| Molecular Weight: | 179 kDa |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 1C10 |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Cytoplasmic Domain of Human LRP6 |
| Species Immunized: | Mouse |
| Purification Method: | Protein G Affinity Chromatography |
| Buffer: | PBS, pH 7.4 |
| Tested Applications: | WB (1:500), IP (2ug) |
| Concentration: | 1 mg/mL |
| Storage: | -20C |
| Shipped: | Cold packs |
Western Blot Analysis
Western blot (100µg) and immuno-precipitation (2mg) analysis of total cell lysates from 293T cells utilizing the LRP6 mAb.
- Khan Z, Vijayakumar S, de la Torre TV, Rotolo S, Bafico A. Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor. Mol Cell Biol 27:7291-301 (2007).
- Goel S1, Chin EN, Fakhraldeen SA, Berry SM, Beebe DJ, Alexander CM.Both LRP5 and LRP6 Receptors Are Required to Respond to Physiological Wnt Ligands in Mammary Epithelial Cells and Fibroblasts. J Biol Chem 287:16454-66 (2012).
- van Dinther M, Zhang J, Weidauer SE, Boschert V, Muth EM, Knappik A, de Gorter DJ, van Kasteren PB, Frisch C, Mueller TD, ten Dijke P. Anti-Sclerostin antibody inhibits internalization of Sclerostin and Sclerostin-mediated antagonism of Wnt/LRP6 signaling. PLoS One 8:e62295 (2013).
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