Anti-DYKDDDDK (FLAG) Tag [2G11-C6-G9] Antibody
![Anti-FLAG (DYKDDDDK) Tag [2G11-C6-G9] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/827_200120200126238260.jpg "Anti-DYKDDDDK (FLAG) Tag [2G11-C6-G9] Antibody")
This mouse monoclonal antibody is specific for the DYKDDDDK (FLAG®*) tag epitope.
Highlights:
- Binds to same epitope as Anti-FLAG®* Antibodies
- Can recognize and bind to N- and C- terminal, as well as most internal FLAG®* tags
?Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
From the laboratory of R. Michael Garavito, PhD, Michigan State University.
Part of The Investigator's Annexe program.
This mouse monoclonal antibody is specific for the DYKDDDDK (FLAG®*) tag epitope.
Highlights:
- Binds to same epitope as Anti-FLAG®* Antibodies
- Can recognize and bind to N- and C- terminal, as well as most internal FLAG®* tags
?Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
From the laboratory of R. Michael Garavito, PhD, Michigan State University.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | DYKDDDDK (FLAG®*, N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) |
| Isotype: | IgGa; kappa light chain |
| Clonality: | Monoclonal |
| Clone Name: | 2G11-C6-G9 |
| Reactivity: | FLAG®* (DYKDDDDK) epitope specific |
| Immunogen: | Tetradecapeptide |
| Species Immunized: | Mouse |
| Epitope: | DYKDDDDK (Binds to same epitope as Anti-FLAG®* Antibodies) |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB/Dot blot (1:5000-10000), Immunoaffinity chromatography |
| Storage: | -20C |
| Shipped: | Cold packs |
Western Blot of the Ectodomain of a Human Cell Surface Protein
Antibody 2G11-C6-G9 (60 min, 1:2000 dilution from 1 mg/mL) was used with a goat anti-mouse-HRP conjugate secondary antibody (Bio-Rad; 30 min, 1:2000 dilution). The blot was visualized with 30 min exposure to TMP-stabilized HRP substrate (Promega). The ectodomain of this human cell surface proteion is part of a fusion protein where the FLAG®* epitope is imbedded between large domains.
*FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
If you publish research with this product, please let us know so we can cite your paper.
