SACK-Xs Lig-13 Clonal Adult Rat Cholangiocytic Stem Cells

Growing and expanding primary, differentiated adult cells in culture is difficult and laborious because many differentiated cells can only be maintained in vitro for a limited period of time.  However, for controlled experiments, it may be necessary to maintain a consistent population of cells.

Maintaining a population of adult stem cells (ASCs), allows one to exponentially propagate a population of cells in vitro, and induce differentiation when desired.  This is possible with SACK cell strains and specified cell culture conditions.

SACK ASC cell properties – Long-term propagation in xanthosine (Xs)-supplemented medium

  • Suppressed asymmetric cell kinetics

Differentiated progeny cell properties – Xs-free medium supplemented with 10% rat serum, confluent monolayer; or serum reduction and Xs-free medium supplemented with 10% bovine serum

Biliary Properties

  • Albumin expression, but no secretion
  • Alpha-fetoprotein secretion
  • CK7 expression (biliary epithelial cell marker)
  • Form tubules with polarized cells (e.g., luminal microvilli)

*Additional similar, but less well characterized, independent clonal cell strains are available. For more information, contact us.

Catalog Number Product Size AVAILABILITY Price Qty
EJS004
Strain “SACK-Xs Lig-13” clonal adult rat cholangiocytic stem cells
1 vial In stock
Price: $799.00
Specifications
Product Type: Cell Line
Name: SACK-Xs Lig-13
Cell Type: adult rat cholangiocytic stem cells
Organism: rat
Accession ID: CVCL_5K98
Morphology: epitheliod
Biosafety Level: Bsl-1
Subculturing: Passage 1:5 when cells reach approx. 80% confluence
Growth Conditions: SACK medium - DMEM (4.5mg/ml high glucose) w/ 10% dialyzed FBS and 400?M Xanthosine
Cryopreservation: 70% DMEM (high glucose); 20% dialyzed FBS; 10% DMSO
Source: liver; derived from adult male Fischer 344 rats
Storage: Liquid nitrogen
Shipped: Dry ice
Comments

Replacement of the SACK medium with differentiation conditions allows the expanded tissue stem cells to regain their in vivo homeostatic state of asymmetric self-renewal, which yields cells committed to tissue-specific differentiation.

References
  1. Sherley et al. (2010) Hepatocyte precursor cell lines. Patent No. US 7,645,610 B2.
  2. Paré, J.-F. and Sherley, J. L. (2006) Biological principles for ex vivoadult stem cell expansion, in Curr. Top. in Devel. Biol., ed. G.Schatten, Elsevier, Inc. (San Diego), Vol. 73, pp. 141-171.
  3. Lee et al. (2003) Clonal expansion of adult rat liver epithelial stemcells by suppression of asymmetric cell kinetics (SACK). Biotech. &Bioeng. 83, 760-771.

If you publish research with this product, please let us know so we can cite your paper.

 
Loading...
Loading...