|Cell Type:||adult rat hepatocytic stem cells|
|Subculturing:||Passage 1:5 when cells reach approx. 80% confluence|
|Growth Conditions:||SACK medium - DMEM (4.5mg/ml high glucose) w/ 10% dialyzed FBS and 1 mM Xanthosine|
|Cryopreservation:||70% DMEM (high glucose); 20% dialyzed FBS; 10% DMSO|
|Source:||liver cells from adult male Fischer 344 rats|
Replacement of the SACK medium with differentiation conditions allows the expanded tissue stem cells to regain their in vivo homeostatic state of asymmetric self-renewal, which yields cells committed to tissue-specific differentiation.
- Sherley et al. (2010) Hepatocyte precursor cell lines. Patent No. US 7,645,610 B2.
- Taghizadeh, R. R. and Sherley, J. L. (2008) "CFP and YFP, but notGFP, provide stable fluorescent marking of rat hepatic adult stemcells,"J. Biomed. Biotech, Article ID 453590, doi:10.1155/2008/453590.
- Paré, J.-F. and Sherley, J. L. (2006) Biological principles for exvivo adult stem cell expansion, in Curr. Top. in Devel. Biol., ed. G.Schatten, Elsevier, Inc. (San Diego), Vol. 73, pp. 141-171.
- Lee, et al. (2005) EMP-1 is a junctional protein in a liver stem cellline and in the liver," Biochem. Biophys. Res. Commun., 334, 996-1003.
- Lee et al. (2003) Clonal expansion of adult rat liver epithelial stemcells by suppression of asymmetric cell kinetics (SACK). Biotech. &Bioeng. 83, 760-771.
- Semino et al. (2003) Functional differentiation of hepatocyte-likespheroid structures from putative liver progenitor cells inthree-dimensional peptide scaffolds. Differentiation 71, 262-270.
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