|Cell Type:||neonatal human liver stem cells|
|Subculturing:||Passage 1:5 when cells reach approx. 80% confluence|
|Growth Conditions:||SACK medium - DMEM (4.5mg/ml high glucose) w/ 10% dialyzed FBS and 1.5mM Xanthosine|
|Cryopreservation:||70% DMEM (high glucose); 20% dialyzed FBS; 10% DMSO|
|Source:||liver; 1 year old male donor cells|
Express the epithelial stem cell biomarker LGR5 and the liver stem cell biomarker EpCAM.
Replacement of the SACK medium with differentiation conditions allows the expanded tissue stem cells to regain their in vivo homeostatic state of asymmetric self-renewal, which yields cells committed to tissue-specific differentiation.
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- Paré, J.-F. and Sherley, J. L. (2006) Biological principles for ex vivo adult stem cell expansion, in Curr. Top. in Devel. Biol., ed. G. Schatten, Elsevier, Inc. (San Diego), Vol. 73, pp. 141-171.
- Rambhatla et al.(2005) Immortal DNA strand cosegregation requires p53/IMPDH-dependentasymmetric self-renewal associated with adult stem cells. Cancer Res. 65, 3155-3161.
- Xia, et al. (1989) Isolation of human sebaceous glands and cultivationof sebaceous gland-derived cells in an in vitro model. J. Invest. Dermatol. 93, 315-321.
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