Anti-tdTomato [16D7] Antibody

This antibody was raised against a recombinant protein and recognizes tdTomato.

The tdTomato protein was derived from a monomeric mutant of DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. tdTomato is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).

From a laboratory at The Scripps Research Institute.

Catalog Number Product Size AVAILABILITY Price Qty
Anti-tdTomato [16D7] Antibody, 100ug
100ug 3-5 weeks
Price: $343.00
Product Type: Antibody
Antigen: Raised against a recombinant protein and recognizes tdTomato.
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: 16D7
Species Immunized: Rat
Purification Method: Protein G
Buffer: PBS, pH 7.4
Tested Applications: WB (1:1000), ELISA, IF, IHC (1:100-500), IP (1ug antibody to 100ug total protein)
Concentration: 1.73 mg/mL
Storage: 4C, -20C for long term storage
Shipped: Cold packs

Immunohistochemistry using Anti-tdTomato [16D7] Antibody in a transgenetic mouse, weakly expressing Cre-driven tdTomato.

The Anti-tdTomato [16D7] Antibod with Alexa-555 secondary was used to visualize Cre+ cells for tdTomato expression. The protocol used is as follows: (1) Perfuse mouse with ~ 25 ml ice-cold normal ringer solution with pH 7.4. (2) Perfuse mouse with ~ 50 ml ice-cold fixative consisting of 2% PFA and 15% picric acid with pH = 7.4. (3) After brain extraction, post fix for one hour with 2% PFA + 15% picric acid. (4) Quickly wash brain 3x with liberal amount of PBS, thoroughly removing all fixative. (5) Slice in ice-cold ringer and section at 60 µm. Wash section 3x with 0.01M PBS, 15’ each time. (6) Block for 1 hours with 10% normal goat serum INGS), 3% Bovine serum albumin (BSA), and 0.1% Trixton-X in 0.01M PBS. (7) Incubate primary tdTomato Ab at 1:500 – 1:250 for 24 hours at 4°C on a shaker. (8) Extract primary solution without washing. (9) Incubate for 2 hours in room temperature secondary (Alexa-555) at 1:500 in the following solution made in 0.01M PBS: 1% NGS + 0.01% Triton-X. (10) Wash sections 3x, 15’ each time. (11) Mount section on microscope slide and cover slip using anti-fading preservative (e.g. Vectashield or Fluoromount-G). Image courtesy of Kerafast customer Vivian Hernandez from Dr. C. Savio Chan’s Lab, Northwestern University, submitted February 23, 2015.

From a laboratory at The Scripps Research Institute.
  1. Hernández VM, Hegeman DJ, Cui Q, Kelver DA, Fiske MP, Glajch KE, Pitt JE, Huang TY, Justice NJ, Chan CS. Parvalbumin+ Neurons and Npas1+ Neurons Are Distinct Neuron Classes in the Mouse External Globus Pallidus. J Neurosci. 2015 Aug 26;35(34):11830-47. doi: 10.1523/JNEUROSCI.4672-14.2015. PubMed PMID: 26311767; PubMed Central PMCID: PMC4549397. View Article
  2. Jhaveri DJ, Tedoldi A, Hunt S, Sullivan R, Watts NR, Power JM, Bartlett PF, Sah P. Evidence for newly generated interneurons in the basolateral amygdala of adult mice. Mol Psychiatry. 2017 Aug 15. View Article
  3. Meltzer S, Bagley JA, Perez GL, O'Brien CE, DeVault L, Guo Y, Jan LY, Jan YN. Phospholipid Homeostasis Regulates Dendrite Morphogenesis in Drosophila Sensory Neurons. Cell Rep. 2017 Oct 24;21(4):859-866. View Article

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