Prader-Willi Syndrome [PWS1-7] Human Induced Pluripotent Stem Cells

These induced pluripotent stem cells (iPSCs) have been derived from previously banked fibroblasts from a female individual with Prader-Willi syndrome (PWS). Like the person they were derived from, these iPSCs have an approximately 5 Mb deletion of paternal 15q11.2-q13.1.

Prader-Willi (PWS) is a neurogenetic disorder in which individuals first present with severe hypotonia and infantile failure to thrive, but later develop severe hyperphagia that leads to morbid obesity. They also have small stature, hands, and feet, behavioral problems, and persistent hypotonia. PWS is most often caused by deletion of the paternally-inherited allele of 15q11-q13.

From the laboratories of Stormy J. Chamberlain, PhD and Marc Lalande, PhD, University of Connecticut.

Catalog Number Product Size AVAILABILITY Price Qty
ECN002
Prader-Willi Syndrome [PWS1-7] Human iPSCs, live cells in T25 flask
1 vial 2-3 weeks
Price: $375.00

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Specifications
Name: PWS1-7
Cell Type: Induced pluripotent stem cell (iPSC)
Organism: Human
Accession ID: CVCL_U529
Morphology: Small round cells that grow in colonies, indistinguishable from human embryonic stem cells
Biosafety Level: 2
Subculturing: Manually split no more than 1:5, depending on density of dish
Growth Conditions: hESC medium (DMEM/F12 with 20% Knockout serum replacer, 1X Non-essential amino acids, 1mM L-glutamine, 4ug/mL basic Fibroblast growth factor)
Cryopreservation: 60% DMEM/F12, 30% FBS, 10% DMSO
Source: Fibroblasts
Storage: Cells should be cultured at 37C upon arrival. Cryopreserved cells should be stored in Liquid nitrogen
Shipped: Ambient temperature as live cells in T25 flask
Provider
From the laboratories of Stormy J. Chamberlain, PhD and Marc Lalande, PhD, University of Connecticut.
Comments

These cells have been propagated using irradiated mouse embryonic fibroblasts as a feeder layer. It is recommended to keep the cells on a feeder layer until established.

References
  1. Chamberlain SJ, Chen PF, Ng KY, Bourgois-Rocha F, Lemtiri-Chlieh F, Levine ES, Lalande M. Induced pluripotent stem cell models of the genomic imprinting disorders Angelman and Prader-Willi syndromes. Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17668-73.

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