|Cell Type:||Induced pluripotent stem cell (iPSC)|
|Morphology:||Small round cells that grow in colonies, indistinguishable from human embryonic stem cells|
|Subculturing:||Manually split no more than 1:5, depending on density of dish|
|Growth Conditions:||hESC medium (DMEM/F12 with 20% Knockout serum replacer, 1X Non-essential amino acids, 1mM L-glutamine, 4ug/mL basic Fibroblast growth factor)|
|Cryopreservation:||60% DMEM/F12, 30% FBS, 10% DMSO|
|Storage:||Cells should be cultured at 37C upon arrival. Cryopreserved cells should be stored in Liquid nitrogen|
|Shipped:||Ambient temperature as live cells in T25 flask|
These cells have been propagated using irradiated mouse embryonic fibroblasts as a feeder layer. It is recommended to keep the cells on a feeder layer until established.
- Chamberlain SJ, Chen PF, Ng KY, Bourgois-Rocha F, Lemtiri-Chlieh F, Levine ES, Lalande M. Induced pluripotent stem cell models of the genomic imprinting disorders Angelman and Prader-Willi syndromes. Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17668-73.
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