|Cell Type:||Induced pluripotent stem cell (iPSC)|
|Morphology:||Small round cells that grow in colonies, indistinguishable from human embryonic stem cells|
|Subculturing:||Manually split no more than 1:5, depending on density of dish|
|Growth Conditions:||hESC medium (DMEM/F12 with 20% Knockout serum replacer, 1X Non-essential amino acids, 1mM L-glutamine, 4ug/mL basic Fibroblast growth factor)|
|Cryopreservation:||60% DMEM/F12, 30% FBS, 10% DMSO|
|Storage:||Cells should be cultured at 37C upon arrival. Cryopreserved cells should be stored in Liquid nitrogen|
|Shipped:||Ambient temperature as live cells in T25 flask|
These cells have been propagated using irradiated mouse embryonic fibroblasts as a feeder layer. It is recommended to keep the cells on a feeder layer until established.
- Sun AX, Yuan Q, Tan S, Xiao Y, Wang D, Khoo AT, Sani L, Tran HD, Kim P, Chiew YS, Lee KJ, Yen YC, Ng HH, Lim B, Je HS. Direct Induction and FunctionalMaturation of Forebrain GABAergic Neurons from Human Pluripotent Stem Cells. CellRep. 2016 Aug 16;16(7):1942-53. View Article
- Chamberlain SJ, Chen PF, Ng KY, Bourgois-Rocha F, Lemtiri-Chlieh F, Levine ES, Lalande M. Induced pluripotent stem cell models of the genomic imprinting disorders Angelman and Prader-Willi syndromes. Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17668-73.
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