Anti-ADAM28 (mouse), Cytoplasmic Domain Antibody
This rabbit IgG polyclonal antibody was generated against the GST-ADAM28-cytoplasmic tail and is specific for the cytoplasmic domain of mouse ADAM28.
The ADAM (A Disintegrin And Metalloprotease Domain) family is a unique group of proteins that has the ability to cleave or shed extracellular portions of transmembrane proteins. In addition, ADAMs are involved in cell-cell and cell-matrix interactions (e.g., fertilization, muscle development and neurogenesis).
Based on sequence identity, ADAM28 is more closely related to snake venom metalloproteases (SVMPs) than to presently known ADAMs. Northern blot analysis shows that ADAM28 is expressed in the epididymis, suggesting a possible role in sperm maturation, and in the lung. Mature ADAM28 is presumed to be catalytically active and found mainly on the cell surface.
From the laboratory of Carl P. Blobel, MD, PhD, Hospital for Special Surgery.
Part of The Investigator's Annexe program.
This rabbit IgG polyclonal antibody was generated against the GST-ADAM28-cytoplasmic tail and is specific for the cytoplasmic domain of mouse ADAM28.
The ADAM (A Disintegrin And Metalloprotease Domain) family is a unique group of proteins that has the ability to cleave or shed extracellular portions of transmembrane proteins. In addition, ADAMs are involved in cell-cell and cell-matrix interactions (e.g., fertilization, muscle development and neurogenesis).
Based on sequence identity, ADAM28 is more closely related to snake venom metalloproteases (SVMPs) than to presently known ADAMs. Northern blot analysis shows that ADAM28 is expressed in the epididymis, suggesting a possible role in sperm maturation, and in the lung. Mature ADAM28 is presumed to be catalytically active and found mainly on the cell surface.
From the laboratory of Carl P. Blobel, MD, PhD, Hospital for Special Surgery.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | GST-ADAM28 Cytoplasmic Domain |
| Accession ID: | Q9UKQ2 |
| Molecular Weight: | ~100 kDa |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Mouse |
| Immunogen: | GST-fusion protein with the cytoplasmic domain of mouse ADAM28 |
| Species Immunized: | Rabbit |
| Epitope: | Cytoplasmic domain |
| Purification Method: | Serum, not purified |
| Tested Applications: | WB (1:500) |
| Storage: | -80C |
| Shipped: | Dry ice |
| Antibody | Species Reactivity | Specificity |
| ADAM9 | Human | Cytoplasmic Domain |
| ADAM9 | Mouse | Cytoplasmic Domain |
| ADAM12 | Mouse | Cytoplasmic Domain |
| ADAM15 | Human | Cytoplasmic Domain |
| ADAM15 | Mouse | Cytoplasmic Domain |
| ADAM15 | Human | Extracellular Domain |
| ADAM15 | Mouse | Disintegrin Domain |
| ADAM17 | Mouse | Cytoplasmic Domain |
| ADAM17 | Mouse | Pro domain |
| ADAM19 | Mouse | Cytoplasmic Domain |
| ADAM28 | Mouse | Cytoplasmic Domain |
| ADAM28 | Mouse | Extracellular Domain |
| SNX9 | Mouse | Cytoplasmic Domain |
Western Blot Analysis
Western blot of lysates from COS-7 cells transfected with a pcDNA control and wild-type ADAM28 (WT). All samples were reduced with DTT and separated on a 7.5% (w/v) polyacrylamide gel. Proteins were transferred to nitrocellulose and immunoblotted with ADAM28 cytoplasmic domain antiserum.
Adapted from: Howard L, et al. Biochem J. 2000, 348, 21-27.
- Howard, L.; Maciewicz R. A.; Blobel C. P. Cloning and characterization of ADAM28: evidence for autocatalytic pro-domain removal and for cell surface localization of mature ADAM28. Biochem J. 2000, 348, 21-27.
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