|Antigen:||JARID1B (also referred to as KDM5B, PLU1)|
|Molecular Weight:||176 kDa|
|Purification Method:||Crude serum|
|Tested Applications:||WB, ELISA, IP|
|Storage:||-20C or 4C|
Immunoprecipitation (IP) and IP-Western experiments
A. Performance of JARID1B rabbit anti-sera in immunoprecipitation experiments. SAOS-2 osteosarcoma cells were transfected with a HA-PLU1 overexpression plasmid and immunoprecipitation reactions were performed with antibodies 4439 and 4440 (at 1:1,000 dilution) or the corresponding pre-immune sera overnight at 4°C. Positive control included anti-HA hybridoma 12CA5. Negative controls included unrelevant antibodies 4437 and 4439, their pre-immune antiserum, rabbit IgG, as well as immunoprecipitations performed with lysates from cells transfected with the vector. The immunoprecipitates were separated by 6.25% SDS-PAGE and immunoblotted with the HA antibody 12CA5. The JARID1B -specific band is just below 180 kDa. B. Performance of JARID1B rabbit anti-sera in IP-Western experiments. SAOS-2 osteosarcoma cell lysates were incubated with the indicated antibodies (4440 was used at 1:1,000 dilution), normalized by protein content, overnight at 4°C. The immunoprecipitates were separated by 6.25% SDS-PAGE and immunoblotted with a different, affinity-purified rabbit KDM5B antibody.
Adapted from: Benevolenskaya EV, unpublished data.
- Islam AB, Richter WF, Lopez-Bigas N, Benevolenskaya EV. Selective targeting of histone methylation. Cell Cycle. 2011 Feb 1;10(3):413-24. PMID: 21270517.
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