|Antigen:||Mu Opioid Receptor (MOP)|
|Clone Name:||NT2 (also known as 5G8)|
|Reactivity:||Human, Mouse, Rat|
|Immunogen:||Multiple antigenic peptide from the N-terminal region of the mu-opioid receptor (sequence: 14SDPLAPASCSPA25)|
|Purification Method:||Protein A|
|Buffer:||100 mM Tris-glycine, pH 7|
|Tested Applications:||WB (1:500-1:1000), ELISA (1:100-1:500), FACS (1:100-1:400)|
|Storage:||-20C. Once thawed, store at 4C (avoid repeat freeze thaw cycles).|
Use of Anti-Mu Opioid Receptor, N-terminal (NT2) Antibody
(A) Western blot with NT2 mAb of CHO cell membranes expressing either mu or delta opioid receptors that were pre-incubated without or with the peptide SDPLAPASCSPA. (B) ELISA with NT2 mAb and CHO cells expressing mu opioid receptors or SKNSH cells that endogenously express mu opioid receptors. (C) ELISA with NT2 mAb and membranes from wild-type (WT) or mu knock-out mice that were treated without or with morphine. (D) FACS analysis with NT2 mAb and CHO cells alone (Top Panel) or CHO cells expressing mu opioid receptors (Bottom Panel) that were treated with the mu opioid receptor agonists DAMGO or morphine.
Adapted from: Gupta A, Décaillot FM, Gomes I, Tkalych O, Heimann AS, Ferro ES, Devi LA. Conformation state-sensitive antibodies to G-protein-coupled receptors. J Biol Chem. 2007 Feb 23;282(8):5116-24.
Important: NT2 mAb exhibits increases in recognition of agonist-treated opioid receptors only when cells/tissues expressing the receptors are treated with the agonists prior to brief fixation with ice-cold methanol.
Gupta A, Décaillot FM, Gomes I, Tkalych O, Heimann AS, Ferro ES, Devi LA. Conformation state-sensitive antibodies to G-protein-coupled receptors. J Biol Chem. 2007 Feb 23;282(8):5116-24.
If you publish research with this product, please let us know so we can cite your paper.