Anti-Glucocorticoid Receptor [FIGR] Antibody
This IgG2b mouse antibody reacts with the glucocorticoid receptor (GR) of mouse and rat, but does not react with human GR; the epitope bound on the mouse receptor is at residues 395 - 411.
The glucocorticoid receptor (GR) is a ubiquitous ligand dependent transcription factor important in cellular and organism hemostasis. It exists in the cytoplasm of virtually all cells in a complex with a number of heat shock, chaperone and immunophilin proteins (1). Upon binding glucocorticoids such as cortisol (endogenous, human)) or dexamethasone (one of many synthetics) the complex is altered through component exchange and migrates into the nucleus where the receptor binds to specific DNA response elements (GRE). Depending on the promoter and co-regulatory molecules present, gene expression can be either induced or repressed (2). In addition the GR can interact with other transcription factors such as NFkB and AP1 to interfere with other signaling pathways. Glucocorticoids are used clinically to modulate diseases or unwanted reactions of the immune system.
From the laboratory of Jack E. Bodwell, PhD, Dartmouth College.
This IgG2b mouse antibody reacts with the glucocorticoid receptor (GR) of mouse and rat, but does not react with human GR; the epitope bound on the mouse receptor is at residues 395 - 411.
The glucocorticoid receptor (GR) is a ubiquitous ligand dependent transcription factor important in cellular and organism hemostasis. It exists in the cytoplasm of virtually all cells in a complex with a number of heat shock, chaperone and immunophilin proteins (1). Upon binding glucocorticoids such as cortisol (endogenous, human)) or dexamethasone (one of many synthetics) the complex is altered through component exchange and migrates into the nucleus where the receptor binds to specific DNA response elements (GRE). Depending on the promoter and co-regulatory molecules present, gene expression can be either induced or repressed (2). In addition the GR can interact with other transcription factors such as NFkB and AP1 to interfere with other signaling pathways. Glucocorticoids are used clinically to modulate diseases or unwanted reactions of the immune system.
From the laboratory of Jack E. Bodwell, PhD, Dartmouth College.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Glucocorticoid Receptor (GR) |
| Accession ID: | P04150, NM_000176 |
| Host: | Mouse |
| Molecular Weight: | 85-100 kDa |
| Isotype: | IgG2b |
| Clonality: | Monoclonal |
| Clone Name: | FIGR |
| Reactivity: | Hamster, Mouse, Rat (does not react with Human) |
| Immunogen: | Native glucocorticoid receptor purified from rat liver |
| Epitope: | the epitope bound on the mouse receptor is at residues 395 - 411 |
| Purification Method: | Protein G |
| Tested Applications: | WB, IP |
| Concentration: | 0.5 mg/mL |
| Storage: | -20C |
| Shipped: | Cold packs |
The antibody produced by FIGR appears to be equivalent to that produced by the BUGR2 hybridoma in that the two antibodies will cross inhibit, and both bind to the same 16,000 dalton tryptic fragment of GR.
- Sanchez ER. Chaperoning steroidal physiology: Lessons from mouse genetic models of Hsp90 and its cochaperones. Biochimica et biophysica acta. 2012;1823(3):722-9. Epub 2011/12/14. doi: 10.1016/j.bbamcr.2011.11.006. PubMed PMID: 22155719; PubMed Central PMCID: PMC3288325.
- Oakley RH, Cidlowski JA. Cellular processing of the glucocorticoid receptor gene and protein: new mechanisms for generating tissue-specific actions of glucocorticoids. J Biol Chem. 2011;286(5):3177-84. Epub 2010/12/15. doi: 10.1074/jbc.R110.179325. PubMed PMID: 21149445; PubMed Central PMCID: PMC3030321.
- Bodwell JE, Ortí E, Coull JM, Pappin DJC, Smith LI, Swift F. Identification of phosphorylated sites in the mouse glucocorticoid receptor. JBiolChem. 1991;266:7549-55.
- Richardson J, Vinson C, Bodwell J. Cyclic adenosine-3',5'-monophsophate-mediated activationof a glutamine synthetase composite glucocorticoid response element. MolEndocrinol. 1999;13(4):546-54.
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