Neutrophil Specific, NIR Fluorescent Imaging Agent
This reagent is a Cyanine7-conjugated, PEG-modified hexapeptide that specifically binds the formylpeptide receptor (FPR) of neutrophils. After tailvein injection of the agent, sites of inflammation, where neutrophils have been recruited and activated, can be imaged in vivo by near-infrared (NIR) fluorescence.
(Left) Representative fluorescence image at 3 hrs post injection of Neutrophil-Specific, NIR Fluorescent Imaging Agent (cFLFLF-PEG76-Cyanine7) (2 nmol): A, normal group; B, blocking group pre-injected with 100 nmol (50 eq.) of a non-fluorescence blocking peptide 1 hr before injection of the Neutrophil-Specific, NIR Fluorescent Imaging Agent.1
From the laboratory of Dongfeng Pan, PhD, University of Virginia.
Read related blog post, Development of a Fluorescent Imaging Probe Specific for Neutrophils »
Part of The Investigator's Annexe program.
|Product: ||Neutrophil-Specific, NIR Fluorescent Imaging Agent (cFLFLF-PEG76-Cyanine7) |
| Molecular Weight: ||5 kDa (average calculated mass) 0.25mg = 50nmol |
| Physical Form: ||lyophilized powder |
| Purity: ||>95%, HPLC |
|Solubility: || Soluble in water (see catalog# CP0691) and PBS (see catalog #: CG1240) |
Create stock solution of 20uM by dissolving 0.25mg into 2.5ml of sterile water or PBS.
|Excitation: ||745nm |
|Emission: ||800nm |
| Applications: ||in vivo imaging |
| Shipped: ||Shipped at room temperature |
|Storage conditions: ||For long term storage, keep at -20C (avoid repeated freeze / thaw cycles) |
Dissolve Neutrophil-Specific, NIR Fluorescent Imaging Agent in water (sterile water see catalog# CP0691) or neutral buffer (PBS see catalog# CG1240) for a working solution concentration of 20uM. Inject mice with 50uL Working Solution per 10g body weight via intravenous injection.
- Inject mice at 2nmol per 20g of body weight.
- The half life of this compound is between 1.5 - 2hrs
Customer Provided Data:
SKH mice from CRL (6 mice per group) were treated with a strong neutrophil-inducing agent. Neutrophil-Specific, NIR Fluorescent Imaging Agent was then dosed at 0.5 nmol per 20g of body weight. Mice were imaged with an IVIS lumina XR imaging system using the auto settings with medium binning and an F stop of 2. Adaptive FL Background subtraction was performed. (Unpublished data from customer at NIH).
- Xiao et al. Synthesis of the Cyanine 7 labeled neutrophil-specific agents for noninvasive near infrared fluorescence imaging. Bioorg. Med. Chem. Lett. 20 (2010) 3515–3517.
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