Low Voltage-Activated (LVA), T-type, Ca+ Channel (Cav3) HEK293 Cell Lines
Three distinct HEK-293 cell lines expressing non-toxic proteins; voltage gated calcium channels called Cav3.1, Cav3.2, or Cav3.3.
Highlights:
- Express low voltage-activated (LVA), T-type, Ca2+ Channel (Cav) 3.1, 3.2, or 3.3
- All cDNAs of human origin
- Cav3.x expression is under G418 selection (0.4 mg/ml maintenance, 1 mg/ml to prepare frozen aliquots)
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The three isoforms, Cav3.1 (alpha-1G), Cav3.2 (alpha-1H), and Cav3.3 (alpha-1I), give rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by mibefradil and various novel blockers (E.g., TTA-P2; Click Here for a complete list). A particularity of this type of channel is an opening at quite negative potentials and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.
From the laboratory of Edward Perez-Reyes, PhD, University of Virginia.
Three distinct HEK-293 cell lines expressing non-toxic proteins; voltage gated calcium channels called Cav3.1, Cav3.2, or Cav3.3.
Highlights:
- Express low voltage-activated (LVA), T-type, Ca2+ Channel (Cav) 3.1, 3.2, or 3.3
- All cDNAs of human origin
- Cav3.x expression is under G418 selection (0.4 mg/ml maintenance, 1 mg/ml to prepare frozen aliquots)
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The three isoforms, Cav3.1 (alpha-1G), Cav3.2 (alpha-1H), and Cav3.3 (alpha-1I), give rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by mibefradil and various novel blockers (E.g., TTA-P2; Click Here for a complete list). A particularity of this type of channel is an opening at quite negative potentials and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.
From the laboratory of Edward Perez-Reyes, PhD, University of Virginia.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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| Product Type: | Cell Line |
| Name: | HEK293 Cav3.1a, Cav3.2a, or Cav3.3b |
| Cell Type: | HEK-293 |
| Accession ID: | Cav3.1a, CVCL_HC62; Cav3.2a, CVCL_HC63; Cav3.3b, CVCL_HC64 |
| Organism: | Human |
| Morphology: | pancake |
| Source: | human embryonic kidney |
| Biosafety Level: | BSL2 |
| Growth Conditions: | D-MEM/F-12 (HyClone DMEM/F-12 1:1 Modified (Cat# SH30261.01). (This medium already has: 2.5 mM L-Glutamine, 15 mM HEPES, 110 mg/L Sodium Pyruvate), 10% FBS, 1% P/S (Penicillin/ Streptomycin; Gibco # 15140-122), Cav3.x expression is under G418 selection (0.4 mg/ml maintenance, 1 mg/ml to prepare frozen aliquots) |
| Subculturing: | 1:10 |
| Cryopreservation: | 5% DMSO, D-MEM/F12, 10% FBS |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
- Perez-Reyes, E., Van Deusen, A., Vitko, I. Molecular Pharmacology of Human Cav3.2 T-Type Ca2+ Channels: Block by Antihypertensives, Antiarrhythmics, and Their Analogs. JPET 328:621-7, 2009.
- Xie X, Van Deusen AL, Vitko I, Babu DA, Davies LA, Huynh N, Cheng H, Yang N,Barrett PQ, Perez-Reyes E. Validation of high throughput screening assays againstthree subtypes of Ca(v)3 T-type channels using molecular and pharmacologicapproaches. Assay Drug Dev Technol. 2007 Apr;5(2):191-203.
- Gomora JC, Murbartián J, Arias JM, Lee JH, Perez-Reyes E. Cloning andexpression of the human T-type channel Ca(v)3.3: insights into prepulsefacilitation. Biophys J. 2002 Jul;83(1):229-41.
- Shin SM, Lauzadis J, Itson-Zoske B, Cai Y, Fan F, Natarajan G, Kwok WM, Puopolo M, Hogan QH, Yu H. Targeting intrinsically disordered regions facilitates discovery of CaV3.2 inhibitory peptides for AAV-mediated peripheral analgesia. Pain. 2022 Apr 14. doi: 10.1097/j.pain.0000000000002650. Epub ahead of print. PMID: 35420557.
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