Anti-NC-37 Cell, NK Cell Mediated Lysis Inhibitory [18C2] Antibody
![Anti-NC-37 Cell,NK Cell Mediated Lysis Inhibitory [18C2] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1244_2001202001264012430.jpg "Anti-NC-37 Cell, NK Cell Mediated Lysis Inhibitory [18C2] Antibody")
This mouse IgM monoclonal antibody was generated against cellular protein and recognizes fish, human and mouse 42kDa protein (and a 86 kDa protein that may be a dimeric form of the first) NC-37 cells (Human B cells).
Highlights:
- Reacts with human, fish and mouse 42kDa protein and 86 kDa protein that may be a dimer from N-37 (human B cell) NK target cells
- Specifically inhibits fish NCC lysis of target cells
- Inhibition of cytotoxicity by mAb 18C2 was dose dependent with inhibition located at the target cell level
- All human or mouse origin target cell lines tested were protected from NCC lysis by prior treatment with this mAb
- Suitable for for Western Blot, Flow Cytometry, ELISA, Immunoprecipitation, Immunofluorescence, Cytotoxicity and Inhibition assays, Immunopanning and FACS applications
NCCs in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/ natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
From a laboratory at University of Georgia.
This mouse IgM monoclonal antibody was generated against cellular protein and recognizes fish, human and mouse 42kDa protein (and a 86 kDa protein that may be a dimeric form of the first) NC-37 cells (Human B cells).
Highlights:
- Reacts with human, fish and mouse 42kDa protein and 86 kDa protein that may be a dimer from N-37 (human B cell) NK target cells
- Specifically inhibits fish NCC lysis of target cells
- Inhibition of cytotoxicity by mAb 18C2 was dose dependent with inhibition located at the target cell level
- All human or mouse origin target cell lines tested were protected from NCC lysis by prior treatment with this mAb
- Suitable for for Western Blot, Flow Cytometry, ELISA, Immunoprecipitation, Immunofluorescence, Cytotoxicity and Inhibition assays, Immunopanning and FACS applications
NCCs in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/ natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
From a laboratory at University of Georgia.
| Product Type: | Antibody |
| Antigen: | 42kDa protein and 86 kDa protein that may be a dimer from N-37 (human B cell) NK target cells |
| Isotype: | IgM |
| Clonality: | Monoclonal |
| Clone Name: | 18C2.8.3 |
| Reactivity: | Porcine, fish, human, hamster, rat, bovine, equine, canine and others |
| Immunogen: | Membrane protein |
| Species Immunized: | Mouse |
| Epitope: | Non-specific cytotoxic cell receptor protein (NCCRP-1) |
| Buffer: | Cell Culture Supernatant |
| Tested Applications: | Flow Cytometry, ELISA, WB, Immunoprecipitation, Cytotoxicity and Inhibition assays, Immunopanning, IF, FACS |
| Storage: | -80 |
| Shipped: | Cold Packs |
- L Jaso-Friedmann, D T Harris, A St John, H S Koren and D L Evans: A monoclonal antibody-purified soluble targetcell antigen inhibits nonspecific cytotoxic cell acitivity. J Immunol 1990; 144:2413-2418.
- D L Evans, L Jaso-Friedmann, E E Smith, Jr, A St John, H S Koren and D T Harris: Identification of a putative antigen receptor on fish nonspecific cytotoxic cells with monoclonal antibodies. J Immunol 1988; 141:324-332.
- L Jaso-Friedmann, D L Evans, C C Grant, A St John, D T Harris and H S Koren: Characterization by monoclonal antibodies of a target cell antigen complex recognized by nonspecific cytotoxic cells. J Immunol 1988; 141:2861-2868.
- D.L. Evans. US Patent 5,194,593 (expired): Antibodies to natural killer cell and non-specific cytotoxic cell receptor and target cell antigens.
- Donald U Evans, David T. Harris, Donna L. Staton, and Liliana Jaso-Friedmann: PATHWAYS OF SIGNAL TRANSDUCTION IN TELEOST NONSPECIFIC CYTOTOXIC CELLS. 1990 Developmental and Comparative Immunology, Vol. 14, pp. 295-304.
- Jaso-Friedmann L,Leary JH,Evans DL: NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells. Molecular immunology , Mar 1998; 34: 955.
- Evans DL,Leary JH,Jaso-Friedmann L: Nonspecific cytotoxic cell receptor protein-1: a novel (predicted) type III membrane receptor on the teleost equivalent of natural killer cells recognizes conventional antigen. Cellular immunology , Jul 1998; 187: 19.
- Viveiros MM,Antczak DF: Characterization of equine natural killer and IL-2 stimulated lymphokine activated killer cell populations. Developmental and comparative immunology, Sep 1999; 23: 521.
- Raymond C,Wilkie B,Mallard B,Quinton M: Natural killer cell frequency and function in Yorkshire pigs selectively bred for high or low antibody and cell-mediated immune response. Natural immunity, Sep 2001; 16: 127.
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