CH12F3 B Lymphocyte Cell Lines
These CH12F3 B lymphocyte cell lines are useful tools to study the role of DNA metabolism in modulating immunoglobulin (Ig) heavy chain class switch recombination (CSR).
CH12F3 is capable of cytokine-induced Ig heavy chain CSR. Key enzymes involved in DNA recombination and joining double strand breaks (DSBs), such as DNA Ligase 1, Ligase 3, Ligase 4 and Apyrimidinic Endonuclease APE1, have been knocked out in these CH12F3 cell lines, providing ideal genetic materials to investigate the roles of these proteins in regulating the proficient CSR event in mature B lymphocyte cell lines. Since enzymes involved in DNA metabolism play essential roles in embryogenesis, knockout of these proteins often resulted in lethality in mouse embryonic stem cells. However, knockout of these proteins in mature B cells are compatible with cell viability.
From the laboratory of Kefei Yu, PhD, Michigan State University.
These CH12F3 B lymphocyte cell lines are useful tools to study the role of DNA metabolism in modulating immunoglobulin (Ig) heavy chain class switch recombination (CSR).
CH12F3 is capable of cytokine-induced Ig heavy chain CSR. Key enzymes involved in DNA recombination and joining double strand breaks (DSBs), such as DNA Ligase 1, Ligase 3, Ligase 4 and Apyrimidinic Endonuclease APE1, have been knocked out in these CH12F3 cell lines, providing ideal genetic materials to investigate the roles of these proteins in regulating the proficient CSR event in mature B lymphocyte cell lines. Since enzymes involved in DNA metabolism play essential roles in embryogenesis, knockout of these proteins often resulted in lethality in mouse embryonic stem cells. However, knockout of these proteins in mature B cells are compatible with cell viability.
From the laboratory of Kefei Yu, PhD, Michigan State University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Cell Line |
| Name: | CH12F3 (APE1-/-/-APE2-) Cell Line |
| Cell Type: | B lymphocyte |
| Accession ID: | P27695, Q59KZ1 |
| Morphology: | Lymphoblast |
| Organism: | Mus musculus |
| Source: | Lymph Node |
| Biosafety Level: | BSL2 |
| Subculturing: | Grow in suspension; 1:5 split every 2-3 days |
| Growth Conditions: | RPMI1640 supplemented with 10% FBS and 50µM 2-Mercaptoethanol |
| Cryopreservation: | Growth medium plus 10% DMSO |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
- Masani S, Han L, Meek K, Yu K. Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination. Proc Natl Acad Sci U S A. 2016, 113(5):1261-6.
- Han L, Masani S, Hsieh CL, Yu K. DNA ligase I is not essential for mammalian cell viability. Cell Rep. 2014 Apr 24;7(2):316-20.
- Masani S, Han L, Yu K. Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination. Mol Cell Biol. 2013 Apr;33(7):1468-73. doi: 10.1128/MCB.00026-13.
- Han L, Masani S, Yu K. Cutting edge: CTNNBL1 is dispensable for Ig classswitch recombination. J Immunol. 2010 Aug 1;185(3):1379-81.
- Han L, Yu K. Altered kinetics of nonhomologous end joining and class switch recombination in ligase IV-deficient B cells. J Exp Med. 2008 Nov 24;205(12):2745-53.
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