pMocr (6xHis-Mocr tag) Plasmid
The pMocr plasmid utilizes an engineered monomeric form of the Ocr protein from bacteriophage T7, which displays solubilizing activity when used as a fusion tag.
Highlights:
- Mocr tag displays solubilizing activity with problematic passenger proteins
- Compatible with high-throughput cloning and expression using ligation independent cloning (LIC)
- N-terminal 6xHis tag and Mocr tag allow for purification via Nickel and DEAE resins, respectively
- Tags are removable via TEV protease cleavage site
The bacteriophage T7 0.3 protein (Ocr for ability to overcome classical restriction) is a 13.8 kDa, highly charged, very acidic (pI = 3.8) protein. It has an efficiently translated transcript and is tolerated at high levels in the cell. There are no cysteines in the Ocr protein. The protein is completely soluble even when expressed to high level and is soluble in 95% ethanol. The Ocr protein may be separated to high levels of purity from E. coli using DEAE resins.
From the laboratory of W. Clay Brown, PhD, University of Michigan.
The pMocr plasmid utilizes an engineered monomeric form of the Ocr protein from bacteriophage T7, which displays solubilizing activity when used as a fusion tag.
Highlights:
- Mocr tag displays solubilizing activity with problematic passenger proteins
- Compatible with high-throughput cloning and expression using ligation independent cloning (LIC)
- N-terminal 6xHis tag and Mocr tag allow for purification via Nickel and DEAE resins, respectively
- Tags are removable via TEV protease cleavage site
The bacteriophage T7 0.3 protein (Ocr for ability to overcome classical restriction) is a 13.8 kDa, highly charged, very acidic (pI = 3.8) protein. It has an efficiently translated transcript and is tolerated at high levels in the cell. There are no cysteines in the Ocr protein. The protein is completely soluble even when expressed to high level and is soluble in 95% ethanol. The Ocr protein may be separated to high levels of purity from E. coli using DEAE resins.
From the laboratory of W. Clay Brown, PhD, University of Michigan.
| Product Type: | Plasmid |
| Gene/insert name: | None |
| Antibiotic Resistance: | Ampicillin |
| Fusion Tag(s): | N-His-Mocr-TEV |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Ampicillin |
| Cloning Site 5': | LIC |
| Cloning Site 3': | LIC |
| Vector Backbone and Size: | pMCSG7 |
| High or low copy: | Medium |
| Storage: | -20C |
| Shipped: | Ambient temperature as liquid or spotted on filter paper |
- DelProposto J, Majmudar CY, Smith JL, Brown WC. Mocr: a novel fusion tag for enhancing solubility that is compatible with structural biology applications. Protein Expr Purif. 2009 Jan;63(1):40-9.
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