Anti-Tartrate Resistant Acid Phosphatase (TRAP/ACP5) Antibodies
This range of mouse monoclonal antibodies was generated against tartrate resistant acid phosphatase (TRAP) and recognizes epitopes throughout TRAP.
Tartrate-resistant acid phosphatase (TRAP) is a glycosylated monomeric metalloprotein enzyme commonly expressed in mammals, that can be used as a biomarker for multiple clinical and basic applications. It has been used as cytochemical marker to identify hairy cell leukemia (HCL) cells as well as a marker for differentiated cells of monocyte lineage such as macrophages, osteoclasts and dendritic cells. TRAP is also used as a serum biomarker for bone disease by measuring elevated levels released from osteoclasts during bone resorption. Other increases in TRAP expression can conclude Gaucher's disease, HIV-induced encephalopathy, and osteoporosis. TRAP consists of two isoforms (Type 5a and 5b) that have different biochemical, clinical and biological properties.
From the laboratory of Anthony Janckila, PhD, University of Louisville
Part of The Investigator's Annexe program.
This range of mouse monoclonal antibodies was generated against tartrate resistant acid phosphatase (TRAP) and recognizes epitopes throughout TRAP.
Tartrate-resistant acid phosphatase (TRAP) is a glycosylated monomeric metalloprotein enzyme commonly expressed in mammals, that can be used as a biomarker for multiple clinical and basic applications. It has been used as cytochemical marker to identify hairy cell leukemia (HCL) cells as well as a marker for differentiated cells of monocyte lineage such as macrophages, osteoclasts and dendritic cells. TRAP is also used as a serum biomarker for bone disease by measuring elevated levels released from osteoclasts during bone resorption. Other increases in TRAP expression can conclude Gaucher's disease, HIV-induced encephalopathy, and osteoporosis. TRAP consists of two isoforms (Type 5a and 5b) that have different biochemical, clinical and biological properties.
From the laboratory of Anthony Janckila, PhD, University of Louisville
Part of The Investigator's Annexe program.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Tartrate Resistant Acid Phosphatase (TRAP/ACP5) |
| Accession ID: | P13686, NM_001111035 |
| Molecular Weight: | 35 kDa |
| Isotype: | IgG2b, kappa |
| Clonality: | Monoclonal |
| Clone Name: | 9C5 |
| Reactivity: | Human |
| Immunogen: | Heat + SDS denatured natural protein from HCL spleen |
| Species Immunized: | Mouse |
| Epitope: | 16kDa subunit |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.02% NaN3 |
| Tested Applications: | WB, IHC w/heat retrieval |
| Comments: | Reactive only with denatured protein of both 5a and 5b isoforms. |
| Storage: | -20C |
| Shipped: | Cold packs |
Tartrate Resistant Acid Phosphatase (TRAP/ACP5) Antibodies
| Clone Name: | 9C5 | 14G6 | 89 | 162 | 203 | 220 |
| Antigen Name: | Tartrate Resistant Acid Phosphatase (TRAP/ACP5) | Tartrate Resistant Acid Phosphatase (TRAP/ACP5) | Tartrate Resistant Acid Phosphatase (TRAP/ACP5) | Tartrate Resistant Acid Phosphatase (TRAP/ACP5) | Tartrate Resistant Acid Phosphatase (TRAP/ACP5) | Tartrate Resistant Acid Phosphatase (TRAP/ACP5) |
| Acession ID: | NM_001111035 | NM_001111035 | NM_001111035 | NM_001111035 | NM_001111035 | NM_001111035 |
| Molecular Weight: | 35 kDa | 35 kDa | 35 kDa | 35 kDa | 35 kDa | 35 kDa |
| Immunogen Isotype: | IgG2b, kappa | IgG1, kappa | IgG | IgG | IgG | IgG |
| Clonality: | Monoclonal | Monoclonal | Monoclonal | Monoclonal | Monoclonal | Monoclonal |
| Species Reactivity: | Human | Human | Human | Human | Human | Human |
| Epitope: | 16kDa subunit | Conformational | Central ?loop peptide? | Conformational | Conformational | Central ?loop peptide? |
| Species Immunized: | Mouse | Mouse | Mouse | Mouse | Mouse | Mouse |
| Immunogen: | Heat + SDS denatured natural protein from HCL spleen | Native natural protein from HCL spleen | Recombinant whole human TRAP | Recombinant whole human TRAP | Recombinant whole human TRAP | Recombinant whole human TRAP |
| Purification Method: | Protein G | Protein G | Protein G | Protein G | Protein G | Protein G |
| Storage Buffer: | PBS, 0.02% NaN3 | |||||
| Tested Applications: | WB, IHC w/heat retrieval | IP, EIA as capture antibody | WB, IHC with heat retrieval, IP, EIA capture antibody | IP, EIA capture or detection antibody | IP, EIA capture or detection antibody | WB, IHC with heat retrieval, IP, EIA capture antibody |
| Storage Temperature: | -20C | -20C | -20C | -20C | -20C | -20C |
| Shipped: | Cold packs | Cold packs | Cold packs | Cold packs | Cold packs | Cold packs |
| Comments: | Reactive only with denatured protein of both 5a and 5b isoforms. | Reactive only with conformationally native protein of both 5a and 5b isoforms. | Reactive only with isoform 5a. Activates enzyme activity and increases pH optimum. May be same or similar epitope as mAb220 with lower sensitivity for EIA. | Reactive only with conformationally native protein of both 5a and 5b isoforms. | Reactive only with conformationally native protein of both 5a and 5b isoforms. May be partially inhibitory to enzyme activity. | Reactive only with isoform 5a. Activates enzyme activity and increases pH optimum. |
mAb Recognition Western Blots and Immunoprecipitates
A: Western blots of dendritic cell lysates labeled with anti-TRAP mABs showing isoform specificity mAb 220 and 89 bound specifically to isoform 5a, where as 95 bound to both isoforms (35kDa 5a band and 16 kDa 5b band). MAb 15G6, 162 and 203 were unreactive. The uniform high-MW bands are due to non-specific streptavidin - biotin interactions and serve as a loading control. B: Immunoprecipitates of dendritic cell lysates using anti-TRACP mABs subjected to western blot and detected with mAb9C5. MAb14G6, 203, 163 under native conditions, precipated both isoforms. 220 and 89 under native conditions precipitated only isoform 5a. 9C5 reacts with denatured epitope of TRAP, showing reactive with TRAP5a-like precursor.
Adapted from: Pradella SD, et al. J Immunol Methods. 2011 Sep 30;372(1-2):162-70.
- Pradella SD, Slone SP, Wu YY, Chao TY, Parthasarathy RN, Yam LT, Janckila AJ. Applications and performance of monoclonal antibodies to human tartrate resistant acid phosphatase. J Immunol Methods. 2011 Sep 30;372(1-2):162-70.
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