Semkur-IM
Semkur-IM is a redox dye for easily assaying cell vitality in real-time.
Highlights:
- Cell permeable and non-toxic
- Negligible effect on GSH levels
- Use with common fluorescent optics
Semkur-IM permeates live cells and fluoresces when the quenching component is cleaved off by cellular glutathione. Fluorescence intensity correlates with reduced glutathione (GSH) an indicator of cellular stress. The reduced/oxidized ratio (redox state) of glutathione typically exceeds 10:1 in healthy cells. Abnormal levels are associated with numerous pathologies including cancer, neurodegenerative diseases, cystic fibrosis, cardiovascular diseases, HIV and aging.
Semkur-IM is a redox dye for easily assaying cell vitality in real-time.
Highlights:
- Cell permeable and non-toxic
- Negligible effect on GSH levels
- Use with common fluorescent optics
Semkur-IM permeates live cells and fluoresces when the quenching component is cleaved off by cellular glutathione. Fluorescence intensity correlates with reduced glutathione (GSH) an indicator of cellular stress. The reduced/oxidized ratio (redox state) of glutathione typically exceeds 10:1 in healthy cells. Abnormal levels are associated with numerous pathologies including cancer, neurodegenerative diseases, cystic fibrosis, cardiovascular diseases, HIV and aging.
Specifications
| Product Type: | Small Molecule |
| Name: | Semkur-IM |
| Alternative Name(s): | DSSQ-1 probe |
| Molecular Weight: | 792 |
| Format: | Liquid |
| Purity: | 99% via HPLC |
| Buffer: | DMSO |
| Ex/Em Maxima: | 485-495/516-525 |
| Platform: | Microplate reader |
| Compatible Cells: | NIH3T3 cells; cultured human cells; plasma; reacts blood components |
| Detection Method: | Fluorescence |
| Comments: | 1mg (sufficient for a 96 well plate) |
| Storage: | Room temperature (powder); -20C resuspended |
| Shipped: | Cold packs |
Documentation
Provider
From a laboratory at Vivid Microscopy.
Comments
The fluorescent probe is a di-thiol based indicator of oxidative stress. Data demonstrates penetration into the mitochondria; however, no penetration into the nuclear membrane. This was determined by co-localization studies.
References
- Alvarez R, Kurfis J, Hendrickson M, Sem DS. Real-time thiol detection in iPSC-derived neuron cultures using SemKur-IM, a novel fluorescent dithio probe. SLAS Discov. 2023 Nov 20:S2472-5552(23)00081-3. doi: 10.1016/j.slasd.2023.11.003. Epub ahead of print. PMID: 38000947.
- Pullela PK, Chiku T, Carvan MJ and Sem D “Fluorescence-based detection of thiols in vitro and in vivo using dithiol probes” Analytical Biochemistry; 352 (2006) 265-273
- Chiku T, Pullela PK and Sem D “A Dithio-Coupled Kinase and ATPase Assay” Journal of Biomolecular Screening 11(7); 2006
- Tracy EP, Dukes M, Beare J, Rowe G, Nair R and LeBlanc AJ. Stromal Vascular Fraction Restores Vasodilatory Function by Reducing Oxidative Stress in Aging-Induced Coronary Microvascular Disease. Antioxid Redox Signal 2022 Aug 11. doi: 10.1089/ars.2021.0249. Online ahead of print
If you publish research with this product, please let us know so we can cite your paper.
