Anti-Rhamnogalacturonan I (Unbranched) Backbone [CCRC M14] Antibody (supernatant)
![Anti-Rhamnogalacturonan I (Unbranched) Backbone [CCRC M14] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1225_2001202001263912240.jpg "Anti-Rhamnogalacturonan I (Unbranched) Backbone [CCRC M14] Antibody (supernatant)")
This mouse IgM monoclonal antibody was generated against sycamore RG-I/MeBSA non and recognizes sycamore and Arabidopsis Rhamnogalacturonan I (RG1).
Highlights:
- Reacts with sycamore and Arabidopsis Rhamnogalacturonan I backbone
- Binds to the backbone of unbranched RG1
- Binding is not affected by the nature of the glycosyl residue at the non-reducing terminus
- Binds moderately to a RG1 backbone oligosaccharide of DP = 9 carrying a single disaccharide branch
- Suitable for ELISA applications for enzymatic studies in plants, biomass deconstruction and characterization and in biofuels research and, together with other antibodies from the same researchers, comprise a biomass deconstruction characterization kit
Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of a-D-GalpA-(1,2)-a-L-Rhap-(1).
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
This mouse IgM monoclonal antibody was generated against sycamore RG-I/MeBSA non and recognizes sycamore and Arabidopsis Rhamnogalacturonan I (RG1).
Highlights:
- Reacts with sycamore and Arabidopsis Rhamnogalacturonan I backbone
- Binds to the backbone of unbranched RG1
- Binding is not affected by the nature of the glycosyl residue at the non-reducing terminus
- Binds moderately to a RG1 backbone oligosaccharide of DP = 9 carrying a single disaccharide branch
- Suitable for ELISA applications for enzymatic studies in plants, biomass deconstruction and characterization and in biofuels research and, together with other antibodies from the same researchers, comprise a biomass deconstruction characterization kit
Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of a-D-GalpA-(1,2)-a-L-Rhap-(1).
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
Specifications
| Product Type: | Antibody |
| Antigen: | Rhamnogalacturonan-I backbone |
| Isotype: | IgM |
| Clonality: | Monoclonal |
| Clone Name: | 10D8.D5.G1 |
| Reactivity: | Sycamore, Arabidopsis, other plant species RG1 |
| Immunogen: | Sycamore RG-I/MeBSA non |
| Species Immunized: | Mouse |
| Epitope: | 2-alpha-Rha1-4-alpha-GalA1-2-alpha-Rha1-4-alpha-GalA1-2-alpha- |
| Buffer: | Cell culture supernatant |
| Tested Applications: | ELISA |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
Documentation
Notes
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
Provider
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
References
- Robin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn and A. Lacey Samuels. 2008. Analysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich Mucilage. The Plant Cell June 2008 vol. 20 no. 6 1623-1638 .
- DeMartini, JD, Pattathil, S, Avci, U, Szekalski, K, Mazumder, K, Hahn, M.G., Wyman, CE: Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production. Energy Environ. Sci., 2011, 4, 4332-4339.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- A.P. de Souza, D.C.C. Leite, S. Pattahil, M.G. Hahn, M.S. Buckeridge. 2013. Composition and structure of sugarcane cell wall polysaccharides: Implications for second generation bioethanol production. Bioenergy Research 6: 564-579.
- J. Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1,2)-linked fucosyl-containing epitope. Plant Physiol. 104:699-710.
- G. Freshour, R. P. Clay, M. S. Fuller, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1996) Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol. 110:1413-1429.
- G. Freshour, C. P. Bonin, W.-D. Reiter, P. Albersheim, A. G. Darvill, and M. G. Hahn. (2003) Distribution of fucose-containing xyloglucans in cell walls of the mur1 mutant of Arabidopsis thaliana. Plant Physiol. 131:1602-1612.
- Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
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