Fluorescent Dye Conjugates from Click Chemistry Tools

Click Chemistry Tools

Fluorescent Dye Conjugates from Click Chemistry Tools

The term “click chemistry” was coined to describe a class of efficient and selective reactions that can be used to join molecules together rapidly and in high yield. This class of chemical reactions uses bio-orthogonal or biologically unique moieties to label and join two molecules of interest in a two-step procedure.

By labelling a biological moiety with a fluorescent dye, one can track the progress of the biomolecule within a particular environment and also observe interactions with other entities using an array offluorescent technologies.

Chemoselective Ligation via Copper-free Click Chemistry


Fluorescent Dye Conjugates
	Dibenzylcyclooctyne (DBCO)-Based Chemical Modification Reagents Combine the advantage of the Cu(I)-catalyzed cycloaddition reaction with the exceptional biocompatibility of the Staudinger ligation.
	Dibenzylcyclooctyne (DBCO) Biotinylation Reagents For the detection of azides in wide range of bioorthogonal reporter applications where the presence of cytotoxic Cu catalyst is unacceptable.
	Dibenzylcyclooctyne (DBCO)-Based PEGylation Reagents For PEGylation of azide-modified biopolymer via Cu-free click reaction.
	Alkyne Reagents These alkyne-fluorescent dye conjugates feature hydrophilic PEG spacers for enhanced solubility in water as well as in commonly used organic solvents of moderate polarity.
	Azide Reagents These azide-fluorescent dye conjugates feature hydrophilic PEG spacers and can be efficiently used for detection of terminal and strained acetylens over a wide range of applications.

References

1. Marks, I. S., et. al. (2011). Strain-Promoted “Click” Chemistry for Terminal Labeling of DNA. Bioconjugate Chem., 22 (7): 1259–63.
van Delft, P., et. al. (2010). Synthesis of Oligoribonucleic Acid Conjugates Using a Cyclooctyne Phosphoramidite. Org. Lett., 12 (23): 5486-89.