Fluorescent Dyes and Click Chemistry Reagents from Lumiprobe
Cyanine (Cy) reagents have been shown to be useful as flluorescent labels for biological compounds. These dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores and allowing for the convenient labeling of antibodes, proteins, drugs, and oligonucletides.
By labelling a biological moiety with a fluorescent dye, one can track the progress of the biomolecule within a particular environment and also observe interactions with other entities using an array offluorescent technologies.
The Cy reagents supplied here include fully customizable azides and alkynes as well as monofunctional NHS-esters, hydrazides, maleimides, and carboxylic acids and are provided in a dried, pre-measured form ready for the labelling of compounds containing free amino groups, carbonyl groups and thiol groups. Additionally, a variety of products suitable for nucleic acid staining in and labeling are also avaliable.
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Click Chemistry Reagents
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Azides
These azides can be used as reagents for Click Chemistry labeling of alkyne-containing molecules.
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Alkynes
Alkynes of fluorescent dyes for Click Chemistry attachment to biomolecules containing reactive azide groups.
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Catalysts and Activators
Copper(II)-TBTA complex and ascorbic acid activator for click chemistry reactions.
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 Click-Chemistry Overview
Click-Chemistry Labeling of Oligonucleotides and DNA
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| Reactive Dyes |
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Dye Activated Esters (amino reactive)
The ester dyes allow for efficient linking to either nucleic acids or proteins via their amino groups.
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Dye Activated Hydrazides (carbonyl reactive)
The hydrazide dyes allow for the labeling of molecules contain carbonyl groups, especially aldehydes. Additionally, hydrazide-containing cy dyes also may be used to detect glycoprotein in cells, tissues, gells or on western blots after periodate oxidation.
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Dye Activated Maleimides (thiol reactive)
The maleimide dyes allow for the selective labeling of sulfhydryl groups such as cysteine residues in proteins and pepties, and oligonucleotides
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Dye Carboxylic Acids (unactivated)
Cy fluorophores are a popular choice for labelling in a variety of fluorescence based applications including drug screening and assay development.
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SYBR Green I
SYBR Green I is an asymmetrical cyanine dye suitable for nucleic acid staining in molecular biology. Once bound to DNA, the dye absorbs blue light and emits green light. SYBR Green I offers an alternative to the traditionally used ethidium bromide tag.
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2D DIGE Kits
These kits are specially designed for precise comparison of protein expression in two or three samples of lysates. Perfect mobility matching and bright dye fluorescence allows for high efficient detection and high resolution of minor proteins on 2D gel electrophoresis.
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Modifed Triphosphates
These terminator triphosphates are suitable for enzymatic end-labeling of DNA. The nucleotide is incorporated by Taq DNA polymerase, Sequenase, Klenow fragment. Subsequent reaction of amino-DNA with activated esters can be used for the non-radioactive end-labeling of DNA.
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References
