
FAST Slide Protein Microarray from Maine Manufacturing
FAST Slides are the premier surface for protein microarray applications, produced by the original FAST team (Whatman/Schleicher & Schuell) now located at Maine Manufacturing. Protein microarrays are tools that can be used in many different areas of research, including basic and translational research.
The most significant advantage of FAST Slides over modified glass surfaces is that the matrix retains arrayed proteins in near-quantitative fashion. This property translates into antibody arrays with unparalleled sensitivity down to antigen concentrations of 1 pg/ml. These qualities make FAST Slides the most reliable surface for microarray experiments and provide a high level of confidence. FAST Slides are compatible with fluorescent, chemiluminescent or radiographic detection systems.
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Protein Arrays |
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Antibody Arrays |
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The high binding capacity, sensitivity and reproducibility of FAST Slides make them ideal for Reverse Phase Protein Arrays (RPPAs) used for biomarker discovery and characterization and in clinical trials. FAST Slides allow quantitative binding across the broad dynamic range of protein concentration found in complex biological samples. The expression of specific proteins is detected with antibodies to the biomarkers of interest.
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The high reproducibility and microporous structure of FAST Slides make themexcellent for protein arrays used to diagnose infection and autoimmune diseasesand for vaccine development and immunity monitoring. In protein arrays, a purifiedprotein is spotted on the FAST Slide surface and the array is used to detectthe presence of antibodies or other binding proteins in clinical or experimental samples.
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FAST Slides are arrayed with multiple antibodies and the presence of specificproteins is detected by applying a complex biological sample to the slide. When used in combination with a standard curve, the unsurpassed binding capacity thatretains the native conformation of proteins bound on FAST Slides allows detectionand quantitation of multiple proteins in a single sample.
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FAST® Slides (Protein Array Surfaces)
FAST Slides are the premier surface for protein microarray applications. FAST Slides are suitable for many types of protein microarrays including antibody arrays and microarray Westerns. There are tremendous advantages to using FAST Slides over traditional ELISAs and Westerns including less sample required, higher sensitivity, linearity and quantitation. The major advantage is that hundreds to thousands of antibodies or samples can be screened simultaneously.
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FAST® PAK (Protein Array Kits)
FAST PAKs (protein array kits) provide all the necessary components for researchers to conveniently build and process their own protein microarrays using the proven FAST technology.
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FAST Quant® (Human Cytokine Assays)
Built on FAST® technology, the high protein binding capacity surface chemistry, FAST Quant® combines the power of array technology with the quantitative nature and high-throughput capabilities of traditional ELISA.
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FAST® Buffers (Optimized Protein Arrays)
KeraFAST protein array reagents have been optimized for use on FAST Slides. These reagents include Single or Two-color Protein Labeling and Fluorescent Detection kits, Protein Arraying Buffer, Protein Array Blocking Buffer, and Protein Array Wash Buffer.
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FAST® Accessories (Protein Array Processing)
Increase the ease and convenience of handling and processing FAST Slides and ensure reproducible, consistent results with every slide, every time.
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FAST® Services (Array Development and Analysis)
KeraFAST is pleased to continue to offer a comprehensive group of protein array services, ranging from contract arraying to slide processing and scanning, all based on the widely accepted FAST Slide protein microarray platform, and provided by same the highly experienced former Whatman FAST team and laboratory.
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Representitive Publications
Alterovitz et al. 2006. Automation, parallelism, and robotics for proteomics. Proteomics 6: 4016-4022.
Angenendt et al. 2003. Next generation of protein microarray support materials: Evaluation for protein and antibody microarray applications. Journal of Chromatography A, 1009: 97-104.
Ho et al. 2006. Linking DNA-binding proteins to their recognition sequences by using protein microarrays. PNAS 103(26): 9940-9945.
Kingsmore et al. 2006. Multiplexed protein measurement: technologies and applications of protein and antibody arrays. Nature Reviews Drug Discovery 5(4): 310-320.
Michaud et al. 2003. Analyzing antibody specificity with whole proteome microarrays. Nature Biotechnology 21: 1509-1512.
Nishizuka et al. 2003a. Proteomic profiling of the NCI-60 cancer cell lines using new high-density reverse-phase lysate microarrays, PNAS 100:14229-14234.
Ramaswamy et al. 2005. Application of Protein Lysate Microarrays to Molecular Marker Verification and Quantification. Proteome Science 2005, 3:9 doi:10.1186/1477-5956-3-9.
Suchyta 2006. Assay: Protein Microarrays on Nitrocellulose Slides. Processes Involved in Using DNA Microarray Instrumentation in Protein Microarray Workflow. Genetic Engineering News 26(2).
Wang et al. 2002. Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells. Nature Biotechnology 20:275-281.
Zhu et al. 2006. Severe acute respiratory syndrome diagnostics using a coronavirus protein array. PNAS 103(11): 4011-4016.